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Analytical methods in strigolactone research

Halouzka, Rostislav ; ZeljkoviÄ, Sanja Äavar ; Klejdus, BoÅivoj ; Tarkowski, Petr

Plant methods, 2020-05, Vol.16 (1), p.1-76, Article 76 [Periódico revisado por pares]

London: BioMed Central Ltd

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Título:
Analytical methods in strigolactone research
Autor: Halouzka, Rostislav ; ZeljkoviÄ, Sanja Äavar ; Klejdus, BoÅivoj ; Tarkowski, Petr
Assuntos: Analytical chemistry ; Analytical methods ; Biological activity ; Biosynthesis ; Botanical research ; Charcoal ; Chemical analysis ; Chromatography ; Determination ; Developmental biology ; Drinking water ; Exudates ; Exudation ; Fungi ; GC–MS ; Germination ; Hormones ; HR-DART-MS ; Isolation ; LC–MS/MS ; Low concentrations ; Phytochemicals ; Plant hormones ; Plant physiology ; Plant tissues ; Researchers ; Review ; Root development ; Roots (Botany) ; Sample preparation ; Solvents ; Strigolactones ; Symbiosis
É parte de: Plant methods, 2020-05, Vol.16 (1), p.1-76, Article 76
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
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ObjectType-Review-1
Descrição: Abstract Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, play important roles in symbioses between higher plants and mycorrhizal fungi, and stimulate germination of plant–parasitic Orobanche and Striga species. Chemical analysis is central to research on the biochemistry of SLs and their roles in developmental biology and plant physiology. Here we summarize key issues relating to the identification and quantification of SLs isolated from plant tissues and exudates. The advantages and drawbacks of different protocols used for strigolactone analysis are discussed, and guidelines for selecting a procedure that will minimize losses during isolation and purification prior to final analysis are proposed. Hyphenated techniques suitable for SL analysis such as GC–MS and LC–MS/MS are also discussed, and newer ambient techniques such as HR-DART-MS and DESI-MS are highlighted as tools with considerable potential in SL research. A key advantage of these methods is that they require only simply sample preparation.
Editor: London: BioMed Central Ltd
Idioma: Inglês

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Analytical methods in strigolactone research

Halouzka, Rostislav ; ZeljkoviÄ, Sanja Äavar ; Klejdus, BoÅivoj ; Tarkowski, Petr

London: BioMed Central Ltd

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