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Effect of Seed Freezing and Storage Conditions on Plasma Membrane Properties of Norway Spruce (Piceo abies Karst.) Seedlings

Rakowski, K. J. ; Behzadipour, M. ; Ratajczak, R. ; Kluge, M.

Botanica Acta, 1998-06, Vol.111 (3), p.236-240 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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  • Título:
    Effect of Seed Freezing and Storage Conditions on Plasma Membrane Properties of Norway Spruce (Piceo abies Karst.) Seedlings
  • Autor: Rakowski, K. J. ; Behzadipour, M. ; Ratajczak, R. ; Kluge, M.
  • Assuntos: fluidity ; H+-ATPase ; Picea abies Karst ; Plasma membrane ; seed storage ; viability
  • É parte de: Botanica Acta, 1998-06, Vol.111 (3), p.236-240
  • Notas: ArticleID:PLB701
    istex:10F912D62D5711F4643156568C45136EC6ECA110
    ark:/67375/WNG-W3JFBQ67-1
  • Descrição: : Norway spruce (Picea abies Karst.) seeds were frozen and stored for 15 months at + 3, − 25, − 75 or − 196°C. After storage, seeds were germinated for 9−14 days to determine viability and plasma membrane protein composition, H+‐ATPase activity and fluidity. The results indicate no significant differences in viability of seed 14 days after germination. Biochemical analyses revealed increased plasma membrane fluidity in 9‐day‐old Norway spruce seedlings raised from seeds pretreated at − 75 °C. and changes in the temperature profile of membrane fluidity in seedlings after pre‐treatment of seeds at − 25 °C. On the other hand, the same treatments did not result in changes in plasma membrane protein content, protein composition or ATPase activity. There was also no difference in plasma membrane H+‐ATPase activity assayed in the presence of different ATP hydrolysis inhibitors. Based on the presented results, and other experimental data, we suggest that during early seedling growth, adaptation of seeds to − 25 and − 75°C freezing and/or storage temperature results in stability of the plasma membrane protein function and composition and increased fluidity or changes in the temperature‐dependent fluidity profile of these membranes.
  • Editor: Oxford, UK: Blackwell Publishing Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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