skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Shifting the Focus from Dissolution to Permeation: Introducing the Meso-fluidic Chip for Permeability Assessment (MCPA)

Tzanova, Martina M. ; Larsen, Bjarke Strøm ; Birolo, Rebecca ; Cignolini, Sara ; Tho, Ingunn ; Chierotti, Michele R. ; Perissutti, Beatrice ; Scaglione, Silvia ; Stein, Paul C. ; Hiorth, Marianne ; Di Cagno, Massimiliano Pio

Journal of pharmaceutical sciences, 2024-05, Vol.113 (5), p.1319-1329 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por
  • Título:
    Shifting the Focus from Dissolution to Permeation: Introducing the Meso-fluidic Chip for Permeability Assessment (MCPA)
  • Autor: Tzanova, Martina M. ; Larsen, Bjarke Strøm ; Birolo, Rebecca ; Cignolini, Sara ; Tho, Ingunn ; Chierotti, Michele R. ; Perissutti, Beatrice ; Scaglione, Silvia ; Stein, Paul C. ; Hiorth, Marianne ; Di Cagno, Massimiliano Pio
  • Assuntos: 2-Methyl-4-chlorophenoxyacetic Acid ; Amorphous solid dispersions ; Animals ; Biopharmaceutics ; Crystalline adducts ; Dissolution/permeation ; Drug Compounding - methods ; Fluidics ; MIVO ; Permeability ; PermeaPad ; Solubility
  • É parte de: Journal of pharmaceutical sciences, 2024-05, Vol.113 (5), p.1319-1329
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: In response to the growing ethical and environmental concerns associated with animal testing, numerous in vitro tools of varying complexity and biorelevance have been developed and adopted in pharmaceutical research and development. In this work, we present one of these tools, i.e., the Meso-fluidic Chip for Permeability Assessment (MCPA), for the first time. The MCPA combines an artificial barrier (PermeaPad®) with an organ-on-chip device (MIVO®) and real-time automated concentration measurements, to yield a sustainable, yet effortless method for permeation testing. The system offers three major physiological aspects, i.e., a biomimetic membrane, an optimal membrane interfacial area-to-donor-volume-ratio (A/V) and a physiological flow on the acceptor/basolateral side, which makes the MPCA an ideal candidate for mechanistic studies and excellent in vivo bioavailability predictions. We validated the method with a handful of assorted drug compounds in unstirred and stirred donor conditions, before exploring its applicability as a tool for dissolution/permeation testing on a BCS class III/I drug (pyrazinamide) crystalline adducts and BCS class II/IV (hydrocortisone) amorphous solid dispersions. The results were highly reproducible and clearly displayed the method's potential for evaluating the performance of enabling formulations, and possibly even predicting in vivo performance. We believe that, upon further development, the MCPA will serve as a useful in vitro tool that could push sustainability into pharmaceutics by refining, reducing and replacing animal testing in early-stage drug development.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês;Norueguês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.