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Tools for large-scale genetic manipulation of yeast genome

Li, Jieyi ; Tong, Hanze ; Wu, Yi

Shengwu gongcheng xuebao, 2023-06, Vol.39 (6), p.2465 [Periódico revisado por pares]

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Título:
Tools for large-scale genetic manipulation of yeast genome
Autor: Li, Jieyi ; Tong, Hanze ; Wu, Yi
Assuntos: Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; Fragments ; Genetic modification ; Genomes ; Nuclease ; Recombinase ; Toolkits ; Translocation ; Yeast ; Yeasts
É parte de: Shengwu gongcheng xuebao, 2023-06, Vol.39 (6), p.2465
Descrição: Large-scale genetic manipulation of the genome refers to the genetic modification of large fragments of DNA using knockout, integration and translocation. Compared to small-scale gene editing, large-scale genetic manipulation of the genome allows for the simultaneous modification of more genetic information, which is important for understanding the complex mechanisms such as multigene interactions. At the same time, large-scale genetic manipulation of the genome allows for larger-scale design and reconstruction of the genome, and even the creation of entirely new genomes, with great potential in reconstructing complex functions. Yeast is an important eukaryotic model organism that is widely used because of its safety and easiness of manipulation. This paper systematically summarizes the toolkit for large-scale genetic manipulation of the yeast genome, including recombinase-mediated large-scale manipulation,nuclease-mediated large-scale manipulation, de novo synthesis of large DNA fragments and other large-sca
Editor: Beijing: Chinese Academy of Sciences
Idioma: Chinês

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