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Direct Observation of Stalled Fork Restart via Fork Regression in the T4 Replication System

Manosas, Maria ; Perumal, Senthil K. ; Croquette, Vincent ; Benkovic, Stephen J.

Science (American Association for the Advancement of Science), 2012-11, Vol.338 (6111), p.1217-1220 [Periódico revisado por pares]

Washington, DC: American Association for the Advancement of Science

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Título:
Direct Observation of Stalled Fork Restart via Fork Regression in the T4 Replication System
Autor: Manosas, Maria ; Perumal, Senthil K. ; Croquette, Vincent ; Benkovic, Stephen J.
Assuntos: ADN ; Analytical, structural and metabolic biochemistry ; Annealing ; Bacteriophage T4 - enzymology ; Bacteriophage T4 - genetics ; Bacteriophages ; Biological and medical sciences ; Collapse ; Damage ; Dealing ; Deoxyribonucleic acid ; DNA ; DNA Damage ; DNA Helicases - metabolism ; DNA Repair ; DNA Replication ; DNA, Cruciform - genetics ; DNA, Viral - genetics ; Enzymes ; Escherichia coli - virology ; Fundamental and applied biological sciences. Psychology ; Genomics ; Holoenzymes - metabolism ; In vitro testing ; Lesions ; Molecular and cellular biology ; Molecular genetics ; Nucleic acids ; Regression ; Reparació de l'ADN ; Replication ; Tweezers ; Viral Proteins - metabolism
É parte de: Science (American Association for the Advancement of Science), 2012-11, Vol.338 (6111), p.1217-1220
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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These authors contributed equally to this work.
Descrição: The restart of a stalled replication fork is a major challenge for DNA replication. Depending on the nature of the damage, different repair processes might be triggered; one is template switching, which is a bypass of a leading-strand lesion via fork regression. Using magnetic tweezers to study the T4 bacteriophage enzymes, we have reproduced in vitro the complete process of template switching. We show that the UvsW DNA helicase in cooperation with the T4 holoenzyme can overcome leading-strand lesion damage by a pseudostochastic process, periodically forming and migrating a four-way Holliday junction. The initiation of the repair process requires partial replisome disassembly via the departure of the replicative helicase. The results support the role of fork regression pathways in DNA repair.
Editor: Washington, DC: American Association for the Advancement of Science
Idioma: Inglês

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Direct Observation of Stalled Fork Restart via Fork Regression in the T4 Replication System

Manosas, Maria ; Perumal, Senthil K. ; Croquette, Vincent ; Benkovic, Stephen J.

Washington, DC: American Association for the Advancement of Science

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