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Laminin-1-enhances secretory activity of a human salivary gland adenoid cystic carcinoma cell line

Moacir Siqueira Junior V. M Freitas; Normando Scarabotto-Neto; N Azambuja Junior; Ruy Gastaldoni Jaeger; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2005

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  • Título:
    Laminin-1-enhances secretory activity of a human salivary gland adenoid cystic carcinoma cell line
  • Autor: Moacir Siqueira Junior
  • V. M Freitas; Normando Scarabotto-Neto; N Azambuja Junior; Ruy Gastaldoni Jaeger; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)
  • Assuntos: HISTOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Objectives: Adenoid cystic carcinoma is a frequent malignant salivary gland neoplasm with high level of recurrence and distant metastasis. We have demonstrated that laminin-1 modulates the phenotype of an adenoid cystic carcinoma (CAC2) cell line. We are currently analyzing the role played by laminin-1 enhancing secretory activity of a CAC2 cells. Methods and results: CAC2 cells were grown inside laminin-1, while cells grown within agarose served as controls. Electron microscopy (EM) showed that CAC2 cells grown within laminin-1 formed pseudocysts filled with secretory-like material. These cells showed also prominent and swollen endoplasmic reticulum, and numerous vesicles. Controls cells, grown in agarose, presented spindle-shaped morphology, with Golgi apparatus, myofilaments and scarce basement membrane. These EM findings suggested that laminin-1 induces secretory activity in CAC2 cells. We further investigated this point by light microscopy, immunofluorescence and confocal microscopy. Histochemistry showed PAS positive material in CAC2 cells treated by laminin. Immunoperoxidase detected type I collagen in these cells. CAC2 cells grown inside agarose (controls) showed no secretory-like material. Confocal microscopy provided additional information on the role played by laminin-1 enhancing secretory activity of CAC2 cells. Whole mount immunofluorescence was carried out in three-dimensional preparations of CAC2 cells grown either within laminin-1 (treated) or
    inside agarose (controls). Optical sections taken 1µm apart showed that laminin-1 enhanced secretion of collagen I and tenascin in CAC2 cells compared to controls. Conclusion: Laminin-1 enhances secretion of type I collagen and tenascin in cells derived from human adenoid cystic carcinoma
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2005
  • Formato: res. 130.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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