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Instantaneous isotropic volumetric imaging of fast biological processes

Wagner, Nils ; Norlin, Nils ; Gierten, Jakob ; de Medeiros, Gustavo ; Balázs, Bálint ; Wittbrodt, Joachim ; Hufnagel, Lars ; Prevedel, Robert

Nature methods, 2019-06, Vol.16 (6), p.497-500 [Periódico revisado por pares]

United States: Nature Publishing Group

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Título:
Instantaneous isotropic volumetric imaging of fast biological processes
Autor: Wagner, Nils ; Norlin, Nils ; Gierten, Jakob ; de Medeiros, Gustavo ; Balázs, Bálint ; Wittbrodt, Joachim ; Hufnagel, Lars ; Prevedel, Robert
Assuntos: Algorithms ; Analysis ; Animals ; Animals, Genetically Modified ; Artefacts ; Biological activity ; Biological systems ; Biophysics ; Blood flow ; Blood Flow Velocity ; Cameras ; Heart ; Heart - diagnostic imaging ; Heart - physiology ; Humans ; Image acquisition ; Image Processing, Computer-Assisted - methods ; Image reconstruction ; Imaging, Three-Dimensional - methods ; Laboratories ; Light ; Medicin och hälsovetenskap ; Methods ; Microscope and microscopy ; Microscopy ; Microscopy - methods ; Molecular biology ; Neurosciences ; Objectives ; Oryzias ; Performance evaluation ; Single-Cell Analysis - methods ; Spatial discrimination ; Spatial resolution ; Three dimensional imaging
É parte de: Nature methods, 2019-06, Vol.16 (6), p.497-500
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.
Editor: United States: Nature Publishing Group
Idioma: Inglês

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Instantaneous isotropic volumetric imaging of fast biological processes

Wagner, Nils ; Norlin, Nils ; Gierten, Jakob ; de Medeiros, Gustavo ; Balázs, Bálint ; Wittbrodt, Joachim ; Hufnagel, Lars ; Prevedel, Robert

United States: Nature Publishing Group

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