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Induction of Inhibitory Factor κ Bα mRNA in the Central Nervous System after Peripheral Lipopolysaccharide Administration: An in situ Hybridization Histochemistry Study in the Rat

Quan, Ning ; Whiteside, Michael ; Kim, Leopold ; Herkenham, Miles

Proceedings of the National Academy of Sciences - PNAS, 1997-09, Vol.94 (20), p.10985-10990 [Periódico revisado por pares]

National Academy of Sciences of the United States of America

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  • Título:
    Induction of Inhibitory Factor κ Bα mRNA in the Central Nervous System after Peripheral Lipopolysaccharide Administration: An in situ Hybridization Histochemistry Study in the Rat
  • Autor: Quan, Ning ; Whiteside, Michael ; Kim, Leopold ; Herkenham, Miles
  • Assuntos: Biological Sciences ; Blood brain barrier ; Blood vessels ; Brain ; Central nervous system ; Choroid plexus ; Cytokines ; Endothelial cells ; Messenger RNA ; Molecules ; Neuroglia
  • É parte de: Proceedings of the National Academy of Sciences - PNAS, 1997-09, Vol.94 (20), p.10985-10990
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
    content type line 23
    To whom reprint requests should be addressed.
    Edited by S. M. McCann, Pennington Biomedical Research Center, Baton Rouge, LA, and approved July 23, 1997
  • Descrição: In this study we investigate the mRNA expression of inhibitory factor κ Bα (Iκ Bα ) in cells of the rat brain induced by an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS). Iκ B controls the activity of nuclear factor κ B, which regulates the transcription of many immune signal molecules. The detection of Iκ B induction, therefore, would reveal the extent and the cellular location of brain-derived immune molecules in response to peripheral immune challenges. Low levels of Iκ Bα mRNA were found in the large blood vessels and in circumventricular organs (CVOs) of saline-injected control animals. After an i.p. LPS injection (2.5 mg/kg), dramatic induction of Iκ Bα mRNA occurred in four spatio-temporal patterns. Induced signals were first detected at 0.5 hr in the lumen of large blood vessels and in blood vessels of the choroid plexus and CVOs. Second, at 1-2 hr, labeling dramatically increased in the CVOs and choroid plexus and spread to small vascular and glial cells throughout the entire brain; these responses peaked at 2 hr and declined thereafter. Third, cells of the meninges became activated at 2 hr and persisted until 12 hr after the LPS injection. Finally, only at 12 hr, induced signals were present in ventricular ependyma. Thus, Iκ Bα mRNA is induced in brain after peripheral LPS injection, beginning in cells lining the blood side of the blood-brain barrier and progressing to cells inside brain. The spatiotemporal patterns suggest that cells of the blood-brain barrier synthesize immune signal molecules to activate cells inside the central nervous system in response to peripheral LPS. The cerebrospinal fluid appears to be a conduit for these signal molecules.
  • Editor: National Academy of Sciences of the United States of America
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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