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Proteome changes associated with Leishmania donovani promastigote adaptation to oxidative and nitrosative stresses

Sardar, Abul Hasan ; Kumar, Sudeep ; Kumar, Ashish ; Purkait, Bidyut ; Das, Sushmita ; Sen, Abhik ; Kumar, Manish ; Sinha, Kislay Kumar ; Singh, Dharmendra ; Equbal, Asif ; Ali, Vahab ; Das, Pradeep

Journal of proteomics, 2013-04, Vol.81, p.185-199 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Proteome changes associated with Leishmania donovani promastigote adaptation to oxidative and nitrosative stresses
Autor: Sardar, Abul Hasan ; Kumar, Sudeep ; Kumar, Ashish ; Purkait, Bidyut ; Das, Sushmita ; Sen, Abhik ; Kumar, Manish ; Sinha, Kislay Kumar ; Singh, Dharmendra ; Equbal, Asif ; Ali, Vahab ; Das, Pradeep
Assuntos: Adaptation, Physiological ; homeostasis ; Humans ; iTRAQ ; LC–MALDI-TOF/TOF-MS ; Leishmania donovani ; Leishmania donovani - metabolism ; macrophages ; menadione ; nitrogen ; oxidative phosphorylation ; Oxidative Stress - drug effects ; Oxidative Stress - physiology ; oxygen ; parasites ; pathogens ; promastigotes ; proteins ; proteome ; Proteome - metabolism ; proteomics ; Proteomics - methods ; Protozoan Proteins - metabolism ; quantitative analysis ; Reactive Nitrogen Species - metabolism ; Reactive Oxygen Species - metabolism ; Redox homeostasis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Superoxide metabolism ; toxicity ; Trypanothione metabolism ; Vitamin K 3 - pharmacology ; Vitamins - pharmacology
É parte de: Journal of proteomics, 2013-04, Vol.81, p.185-199
Notas: http://dx.doi.org/10.1016/j.jprot.2013.01.011
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Descrição: Phagocytic cells produce reactive oxygen and nitrogen species (ROS & RNS) as the most common arsenal to kill intracellular pathogens. Leishmania, an obligate intracellular pathogen also confronts this antimicrobial assault during the early phase of infection but nevertheless is able to survive these attacks and proliferate in macrophage. Adaptation of Leishmania to the toxic effects of ROS and RNS, involves a rapid change in the parasite proteome to combat the host defense response that macrophage mount in combating pathogen. To understand the events associated with combating ROS and RNS species, we performed a proteomic analysis of L. donovani promastigotes treated with sub-lethal doses of menadione (ROS), S-nitroso-N-acetylpenicillamine (RNS) or combination of both compounds. Proteomic changes triggered by these reagents were evaluated by iTRAQ labeling and subsequent LC–MALDI-TOF/TOF-MS analysis. Across the 3 stress conditions, the quantitative analysis identified changes in the proteins which encompass ~20% of the parasite proteome. Major changes were observed in enzymatic machinery of pathways involved in maintaining redox homeostasis, trypanothione metabolism, oxidative phosphorylation, superoxide metabolism, mitochondrial respiration process and other essential metabolic pathways. These observations shed light on how Leishmania promastigotes counter ROS and RNS effects during the initial stage of infection. This article is part of a Special Issue entitled: From protein structures to clinical applications. [Display omitted] ► 20% of the Leishmania donovani proteome identified across the 3 stress conditions ► Parasites reorganize the redox homeostasis and trypanothione metabolism during stress. ► Changes in oxidative phosphorylation, superoxide metabolism and respiration observed ► Maximum changes in the proteome observed upon exposure to peroxynitrile stress ► Explored how L. donovani parasites adapt the stress during early stage of infection
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Proteome changes associated with Leishmania donovani promastigote adaptation to oxidative and nitrosative stresses

Sardar, Abul Hasan ; Kumar, Sudeep ; Kumar, Ashish ; Purkait, Bidyut ; Das, Sushmita ; Sen, Abhik ; Kumar, Manish ; Sinha, Kislay Kumar ; Singh, Dharmendra ; Equbal, Asif ; Ali, Vahab ; Das, Pradeep

Netherlands: Elsevier B.V

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