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Evaluation of a hemolysis-in-gel test for detection and quantitation of antibodies to bluetongue virus [Sheep and calves]

Jochim, M M ; Jones, S C

American journal of veterinary research, 1980-04, Vol.41 (4), p.595-599 [Periódico revisado por pares]

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Título:
Evaluation of a hemolysis-in-gel test for detection and quantitation of antibodies to bluetongue virus [Sheep and calves]
Autor: Jochim, M M ; Jones, S C
Assuntos: Animals ; Antibodies, Viral - analysis ; Bluetongue - diagnosis ; Bluetongue virus - immunology ; Cattle ; Cattle Diseases - diagnosis ; Hemolytic Plaque Technique ; Neutralization Tests ; Reoviridae - immunology ; Sheep ; Sheep Diseases - diagnosis
É parte de: American journal of veterinary research, 1980-04, Vol.41 (4), p.595-599
Notas: L
L73
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Descrição: A hemolysis-in-gel (HIG) test was developed to detect and quantitate antibody to bluetongue virus (BTV). The HIG test was sensitive and accurate when applied to sera from sheep and cattle infected with BTV. Sensitized sheep RBC were prepared by adsorption of partly purified BTV to the cells. Regression analysis of data showed a linear relationship between the diameter of the hemolytic zone and the log of the antibody concentration. The HIG test did not differentiate among antiodies to four serotypes of BTV, but did differentiate between antibody of BTV and antibody to epizootic hemorrhagic disease virus.
Editor: United States
Idioma: Inglês

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