skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Influence of cell-wall thickness on cell division: electron microscopic study with Bacillus cereus

Chung, K. L

Canadian journal of microbiology, 1973-02, Vol.19 (2), p.217-221 [Periódico revisado por pares]

Ottawa, Canada: NRC Research Press

Sem texto completo

Citações Citado por
  • Título:
    Influence of cell-wall thickness on cell division: electron microscopic study with Bacillus cereus
  • Autor: Chung, K. L
  • Assuntos: Bacillus cereus - cytology ; Bacillus cereus - drug effects ; Bacillus cereus - growth & development ; Cell Division ; Cell Membrane ; Cell Wall ; Chloramphenicol - pharmacology ; Culture Media ; Microscopy, Electron ; Models, Biological ; Staining and Labeling ; Time Factors
  • É parte de: Canadian journal of microbiology, 1973-02, Vol.19 (2), p.217-221
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Bacillus cereus that had been exposed to chloramphenicol for 0 and 90 min synthesized walls whose thickness increased with time. These cells were washed and resuspended in fresh growth medium. Cell division was examined by electron microscopy. In untreated cells, a slight invagination of the cytoplasmic membrane with deposit of cross-wall material marked the onset of cell division. The cross wall grew inward until the septum was about completed. This was followed by splitting and bifurcation of the outer edge of the cross wall, which in turn separated the peripheral wall scars. Cross-wall penetration with further splitting continued until the daughter cells were completely partitioned. The peripheral wall scars that were located at the junction of peripheral and end walls at the time of cell separation indicated that the zone of cell division was probably not a region of active cell elongation. In cells treated with chloramphenicol for 90 min, cross-wall initiation and completion all occurred beneath a thickened peripheral wall. The circumferential portion of the peripheral wall at the zone of cell division appeared to be responsible for the hindrance of cell separation. This thick layer subsequently severed at three or four sites to allow the separation of daughter cells. For cells that had initiated cross-wall invagination at the time when chloramphenicol was added, accumulation of a large amount of cross-wall material at the zone of cell division was observed. Upon removal of the antibiotic, cells abandoned the old and initiated a new dividing site. For cells that had already completed cross-wall formation at the time of chloramphenicol treatment, the increase in wall thickness did not appear to interfere with cell separation. Models for the different stages of cell division in all these bacteria were presented.
  • Editor: Ottawa, Canada: NRC Research Press
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.