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Domain alternation and active site remodeling are conserved structural features of ubiquitin E1

Lv, Zongyang ; Yuan, Lingmin ; Atkison, James H. ; Aldana-Masangkay, Grace ; Chen, Yuan ; Olsen, Shaun K.

The Journal of biological chemistry, 2017-07, Vol.292 (29), p.12089-12099 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Domain alternation and active site remodeling are conserved structural features of ubiquitin E1
Autor: Lv, Zongyang ; Yuan, Lingmin ; Atkison, James H. ; Aldana-Masangkay, Grace ; Chen, Yuan ; Olsen, Shaun K.
Assuntos: adenylation ; Amino Acid Substitution ; BASIC BIOLOGICAL SCIENCES ; Catalytic Domain ; conformational change ; Crystallography, X-Ray ; Cysteine - metabolism ; Databases, Protein ; Disulfides - chemistry ; Disulfides - metabolism ; Disulfides - pharmacology ; domain alternation ; Enzyme Activation ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - metabolism ; Enzyme Inhibitors - pharmacology ; enzyme mechanism ; enzyme structure ; Ligands ; Models, Molecular ; Mutation ; Protein Conformation ; Protein Interaction Domains and Motifs ; Protein Multimerization - drug effects ; Protein Processing, Post-Translational - drug effects ; Protein Refolding ; Protein Structure and Folding ; Recombinant Proteins - chemistry ; Recombinant Proteins - metabolism ; Schizosaccharomyces pombe Proteins - antagonists & inhibitors ; Schizosaccharomyces pombe Proteins - chemistry ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Structural Homology, Protein ; structure-function ; SUMO-1 Protein - chemistry ; SUMO-1 Protein - genetics ; SUMO-1 Protein - metabolism ; thioester ; ubiquitin ; Ubiquitin - chemistry ; Ubiquitin - genetics ; Ubiquitin - metabolism ; Ubiquitin-Activating Enzymes - antagonists & inhibitors ; Ubiquitin-Activating Enzymes - chemistry ; Ubiquitin-Activating Enzymes - genetics ; Ubiquitin-Activating Enzymes - metabolism ; Ubiquitin-Conjugating Enzymes - chemistry ; Ubiquitin-Conjugating Enzymes - genetics ; Ubiquitin-Conjugating Enzymes - metabolism ; X-ray crystallography
É parte de: The Journal of biological chemistry, 2017-07, Vol.292 (29), p.12089-12099
Notas: AC02-06CH11357
USDOE Office of Science (SC)
Edited by George N. DeMartino
Descrição: E1 enzymes for ubiquitin (Ub) and Ub-like modifiers (Ubls) harbor two catalytic activities that are required for Ub/Ubl activation: adenylation and thioester bond formation. Structural studies of the E1 for the Ubl small ubiquitin-like modifier (SUMO) revealed a single active site that is transformed by a conformational switch that toggles its competency for catalysis of these two distinct chemical reactions. Although the mechanisms of adenylation and thioester bond formation revealed by SUMO E1 structures are thought to be conserved in Ub E1, there is currently a lack of structural data supporting this hypothesis. Here, we present a structure of Schizosaccharomyces pombe Uba1 in which the second catalytic cysteine half-domain (SCCH domain) harboring the catalytic cysteine has undergone a 106° rotation that results in a completely different network of intramolecular interactions between the SCCH and adenylation domains and translocation of the catalytic cysteine 12 Å closer to the Ub C terminus compared with previous Uba1 structures. SCCH domain alternation is accompanied by conformational changes within the Uba1 adenylation domains that effectively disassemble the adenylation active site. Importantly, the structural and biochemical data suggest that domain alternation and remodeling of the adenylation active site are interconnected and are intrinsic structural features of Uba1 and that the overall structural basis for adenylation and thioester bond formation exhibited by SUMO E1 is indeed conserved in Ub E1. Finally, the mechanistic insights provided by the novel conformational snapshot of Uba1 presented in this study may guide efforts to develop small molecule inhibitors of this critically important enzyme that is an active target for anticancer therapeutics.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Domain alternation and active site remodeling are conserved structural features of ubiquitin E1

Lv, Zongyang ; Yuan, Lingmin ; Atkison, James H. ; Aldana-Masangkay, Grace ; Chen, Yuan ; Olsen, Shaun K.

United States: Elsevier Inc

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