skip to main content
Idioma:

The Engineered Chloroplast Genome Just Got Smarter

Jin, Shuangxia ; Daniell, Henry

Trends in plant science, 2015-10, Vol.20 (10), p.622-640 [Periódico revisado por pares]

England: Elsevier Ltd

Texto completo disponível

Citações Citado por
  • Título:
    The Engineered Chloroplast Genome Just Got Smarter
  • Autor: Jin, Shuangxia ; Daniell, Henry
  • Assuntos: Chloroplasts - genetics ; Gene Expression Regulation, Plant ; Genome, Chloroplast - genetics ; Photosynthesis - genetics ; Plant Leaves - genetics ; Signal Transduction
  • É parte de: Trends in plant science, 2015-10, Vol.20 (10), p.622-640
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-2
    ObjectType-Feature-2
  • Descrição: Chloroplasts are known to sustain life on earth by providing food, fuel, and oxygen through the process of photosynthesis. However, the chloroplast genome has also been smartly engineered to confer valuable agronomic traits and/or serve as bioreactors for the production of industrial enzymes, biopharmaceuticals, bioproducts, or vaccines. The recent breakthrough in hyperexpression of biopharmaceuticals in edible leaves has facilitated progression to clinical studies by major pharmaceutical companies. This review critically evaluates progress in developing new tools to enhance or simplify expression of targeted genes in chloroplasts. These tools hold the promise to further the development of novel fuels and products, enhance the photosynthetic process, and increase our understanding of retrograde signaling and cellular processes. Hyperexpression of biopharmaceuticals in edible leaf chloroplasts documents a recent breakthrough in low-cost oral delivery of biopharmaceuticals that are bio-encapsulated in plant cells. This will enable treating human metabolic or genetic diseases, such as Alzheimer's, diabetes, hypertension, hemophilia, and retinal diseases. New tools for smart chloroplast genome engineering are now available, including Gateway/modular vectors, RNAi interference, and species-specific vectors for efficient transformation of new crop chloroplast genomes and enhanced transgene expression. Multigene metabolic engineering of chloroplasts can be used to produce high value bioproducts. Single chloroplast transgenes are used to confer biotic/abiotic stress tolerance or enhance biomass. Regulation of the nuclear genome is enabled by genes expressed in chloroplasts via retrograde signaling.
  • Editor: England: Elsevier Ltd
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.