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Production of Polygalacturonases by Aspergillus section Nigri Strains in a Fixed Bed Reactor

Marília Maciel ; Cristiane Ottoni ; Cledir Santos ; Nelson Lima ; Keila Moreira ; Cristina Souza-Motta

Molecules, 01 January 2013, Vol.18(2), pp.1660-1671 [Periódico revisado por pares]

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  • Título:
    Production of Polygalacturonases by Aspergillus section Nigri Strains in a Fixed Bed Reactor
  • Autor: Marília Maciel ; Cristiane Ottoni ; Cledir Santos ; Nelson Lima ; Keila Moreira ; Cristina Souza-Motta
  • Assuntos: Aspergillus Niger ; Orange Peel ; Immobilization Cells ; Polygalacturonases ; Fixed Bed Reactor ; Chemistry
  • É parte de: Molecules, 01 January 2013, Vol.18(2), pp.1660-1671
  • Descrição: Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U · mL-1 and 4.11 U · mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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