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New efficient recombinant expression system to engineer Candida antarctica lipase B

Emond, Stéphane ; Montanier, Cédric ; Nicaud, Jean-Marc ; Marty, Alain ; Monsan, Pierre ; André, Isabelle

Applied and Environmental Microbiology 8 (76), 2684-2687. (2010), 2010

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Título:
New efficient recombinant expression system to engineer Candida antarctica lipase B
Autor: Emond, Stéphane ; Montanier, Cédric ; Nicaud, Jean-Marc ; Marty, Alain ; Monsan, Pierre ; André, Isabelle
Assuntos: Biotechnologies
É parte de: Applied and Environmental Microbiology 8 (76), 2684-2687. (2010), 2010
Notas: 10.1128/AEM.03057-09
http://prodinra.inra.fr/ft/2BBFC836-6079-4463-861B-7ECD8B77F9DB
http://prodinra.inra.fr/record/322823
Descrição: Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.
Idioma: Inglês

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