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Ionic liquids as alternative co-solvents for laccase: Study of enzyme activity and stability

Tavares, Ana Paula Mora ; Rodriguez, Oscar ; Macedo, Eugénia A

Biotechnology and bioengineering, 2008-09, Vol.101 (1), p.201-207 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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  • Título:
    Ionic liquids as alternative co-solvents for laccase: Study of enzyme activity and stability
  • Autor: Tavares, Ana Paula Mora ; Rodriguez, Oscar ; Macedo, Eugénia A
  • Assuntos: Biochemistry ; Biological and medical sciences ; Biotechnology ; Chemical bonds ; Computer Simulation ; Enzyme Activation ; Enzyme Stability ; Enzymes ; Fundamental and applied biological sciences. Psychology ; ionic liquids ; Ionic Liquids - chemistry ; kinetic parameters ; Kinetics ; laccase ; Laccase - chemistry ; Liquids ; Models, Chemical ; organic solvents ; Solvents ; Solvents - chemistry ; stability
  • É parte de: Biotechnology and bioengineering, 2008-09, Vol.101 (1), p.201-207
  • Notas: http://dx.doi.org/10.1002/bit.21866
    Fundação para a Ciência e a Tecnologia (FCT, Portugal) - No. SFRH/BPD/22697/2005; No. SFRH/BPD/24271/2005
    ArticleID:BIT21866
    ark:/67375/WNG-GJ4CS7M8-C
    istex:97F9893E9E4286B318F0ACEB00032D1A8B7C2C70
    LSRE - No. FEDER/POCI/2010
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    ObjectType-Article-2
    ObjectType-Feature-1
    content type line 23
  • Descrição: The activity and stability of commercial laccase (DeniLite base) in three different water soluble ionic liquids (ILs) (1-ethyl-3-methylimidazolium 2-(2-methoxyethoxy) ethylsulfate, [emim][MDEGSO₄], 1-ethyl-3-methylimidazolium ethylsulfate, [emim][EtSO₄], and 1-ethyl-3-methylimidazolium methanesulfonate, [emim][MeSO₃]) have been studied and compared to that in two organic solvents (acetonitrile and dimethyl sulfoxide). Initial enzyme activities were similar among the ILs if the same conditions were used. A high reduction on initial enzyme activity was found with acidic pH (5.0). The effect of pH and solvent concentration on enzyme stability were investigated in more detail for 1 week. The enzyme maintained a high stability at pH 9.0 for all ILs tested. [emim][MDEGSO₄] was the most promising IL for laccase with an activity loss of about 10% after 7 days of incubation. The kinetic studies in the presence of ABTS as substrate allowed to calculate the Michaelis- Menten parameters. Good agreement was found between experimental data and calculated values using the Michaelis-Menten mechanism, with a total average relative deviation of 2.1%. Biotechnol. Biotechnol. Bioeng. 2008;101: 201-207.
  • Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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