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Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation

Sibov, Tatiana Taís ; Severino, P. ; Marti, L. C. ; Pavon, L. F. ; Oliveira, D. M. ; Tobo, P. R. ; Campos, A. H. ; Paes, A. T. ; Amaro, E. ; F Gamarra, L. ; Moreira-Filho, C. A.

Cytotechnology (Dordrecht), 2012-10, Vol.64 (5), p.511-521 [Periódico revisado por pares]

Dordrecht: Springer Netherlands

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Título:
Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation
Autor: Sibov, Tatiana Taís ; Severino, P. ; Marti, L. C. ; Pavon, L. F. ; Oliveira, D. M. ; Tobo, P. R. ; Campos, A. H. ; Paes, A. T. ; Amaro, E. ; F Gamarra, L. ; Moreira-Filho, C. A.
Assuntos: Adipocytes ; Adipogenesis ; Biochemistry ; Biological and medical sciences ; Biomedicine ; Biotechnology ; CD105 antigen ; CD73 antigen ; CD90 antigen ; Cell differentiation ; Cell therapy ; Chemistry ; Chemistry and Materials Science ; Cord blood ; Cytology ; DNA microarrays ; Fatty acids ; Flow cytometry ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene regulation ; Mesenchymal stem cells ; Monoclonal antibodies ; Original Research ; Proteins ; Software ; Stem cells ; Umbilical cord
É parte de: Cytotechnology (Dordrecht), 2012-10, Vol.64 (5), p.511-521
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Isolation of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) from full-term deliveries is a laborious, time-consuming process that results in a low yield of cells. In this study we identified parameters that can be helpful for a successful isolation of UCB-MSCs. According to our findings, chances for a well succeeded isolation of these cells are higher when MSCs were isolated from UCB collected from normal full-term pregnancies that did not last over 37 weeks. Besides the duration of pregnancy, blood volume and storage period of the UCB should also be considered for a successful isolation of these cells. Here, we found that the ideal blood volume collected should be above 80 mL and the period of storage should not exceed 6 h. We characterized UCB-MSCs by morphologic, immunophenotypic, protein/gene expression and by adipogenic differentiation potential. Isolated UCB-MSCs showed fibroblast-like morphology and the capacity of differentiating into adipocyte-like cells. Looking for markers of the undifferentiated status of UCB-MSCs, we analyzed the UCB-MSCs’ protein expression profile along different time periods of the differentiation process into adipocyte-like cells. Our results showed that there is a decrease in the expression of the markers CD73, CD90, and CD105 that correlates to the degree of differentiation of UCB-MSCs We suggest that CD90 can be used as a mark to follow the differentiation commitment degree of MSCs. Microarray results showed an up-regulation of genes related to the adipogenesis process and to redox metabolism in the adipocyte-like differentiated MSCs. Our study provides information on a group of parameters that may help with successful isolation and consequently with characterization of the differentiated/undifferentiated status of UCB-MSCs, which will be useful to monitor the differentiation commitment of UCB-MSC and further facilitate the application of those cells in stem-cell therapy.
Editor: Dordrecht: Springer Netherlands
Idioma: Inglês

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Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation

Sibov, Tatiana Taís ; Severino, P. ; Marti, L. C. ; Pavon, L. F. ; Oliveira, D. M. ; Tobo, P. R. ; Campos, A. H. ; Paes, A. T. ; Amaro, E. ; F Gamarra, L. ; Moreira-Filho, C. A.

Dordrecht: Springer Netherlands

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