Idioma:

Convergence of miR-143 overexpression, oxidative stress and cell death in HCT116 human colon cancer cells

Gomes, Sofia E ; Pereira, Diane M ; Roma-Rodrigues, Catarina ; Fernandes, Alexandra R ; Borralho, Pedro M ; Rodrigues, Cecília M P Ahmad, Aamir

PloS one, 2018-01, Vol.13 (1), p.e0191607-e0191607 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Convergence of miR-143 overexpression, oxidative stress and cell death in HCT116 human colon cancer cells
Autor: Gomes, Sofia E ; Pereira, Diane M ; Roma-Rodrigues, Catarina ; Fernandes, Alexandra R ; Borralho, Pedro M ; Rodrigues, Cecília M P
Ahmad, Aamir
Assuntos: Angiogenesis ; Antioxidants ; Apoptosis ; Biological activity ; Biology and Life Sciences ; Cancer ; Cancer therapies ; Cardiomyocytes ; Cell Death ; Cell growth ; Cell Proliferation ; Chemotherapy ; Colon ; Colon cancer ; Colonic Neoplasms - genetics ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Colorectal cancer ; Deregulation ; Gene Expression ; HCT116 Cells ; Human performance ; Humans ; Immunoblotting ; Leukemia ; Medical research ; Medicine and Health Sciences ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Oxaliplatin ; Oxidation resistance ; Oxidative Stress ; Oxygen ; Pathways ; Pharmacology ; Pharmacy ; Protein folding ; Proteins ; R&D ; Reactive oxygen species ; Research & development ; Research and Analysis Methods ; Sensitivity analysis ; Signal transduction ; Signaling ; Superoxide dismutase ; Superoxide Dismutase-1 - genetics ; Tumorigenesis ; Tumors
É parte de: PloS one, 2018-01, Vol.13 (1), p.e0191607-e0191607
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: MicroRNAs (miRNAs) regulate a wide variety of biological processes, including tumourigenesis. Altered miRNA expression is associated with deregulation of signalling pathways, which in turn cause abnormal cell growth and de-differentiation, contributing to cancer. miR-143 and miR-145 are anti-tumourigenic and influence the sensitivity of tumour cells to chemotherapy and targeted therapy. Comparative proteomic analysis was performed in HCT116 human colon cancer cells stably transduced with miR-143 or miR-145. Immunoblotting analysis validated the proteomic data in stable and transient miRNA overexpression conditions in human colon cancer cells. We show that approximately 100 proteins are differentially expressed in HCT116 human colon cancer cells stably transduced with miR-143 or miR-145 compared to Empty control cells. Further, Gene Ontology and pathway enrichment analysis indicated that proteins involved in specific cell signalling pathways such as cell death, response to oxidative stress, and protein folding might be modulated by these miRNAs. In particular, antioxidant enzyme superoxide dismutase 1 (SOD1) was downregulated by stable expression of either miR-143 or miR-145. Further, SOD1 gain-of-function experiments rescued cells from miR-143-induced oxidative stress. Moreover, miR-143 overexpression increased oxaliplatin-induced apoptosis associated with reactive oxygen species generation, which was abrogated by genetic and pharmacological inhibition of oxidative stress. Overall, miR-143 might circumvent resistance of colon cancer cells to oxaliplatin via increased oxidative stress in HCT116 human colon cancer cells.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Convergence of miR-143 overexpression, oxidative stress and cell death in HCT116 human colon cancer cells

Gomes, Sofia E ; Pereira, Diane M ; Roma-Rodrigues, Catarina ; Fernandes, Alexandra R ; Borralho, Pedro M ; Rodrigues, Cecília M P Ahmad, Aamir

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.