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CD8+T cell adjuvant effects ofSalmonellaFliCdflagellin in live vaccine vectors or as purified protein

Braga, Catarina JM ; Massis, Liliana M ; Sbrogio-Almeida, Maria E ; Alencar, Bruna CG ; Bargieri, Daniel Y ; Boscardin, Silvia B ; Rodrigues, Maurício M ; Ferreira, Luís CS

Vaccine, 2010-02, Vol.28 (5), p.1373 [Periódico revisado por pares]

Kidlington: Elsevier Limited

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  • Título:
    CD8+T cell adjuvant effects ofSalmonellaFliCdflagellin in live vaccine vectors or as purified protein
  • Autor: Braga, Catarina JM ; Massis, Liliana M ; Sbrogio-Almeida, Maria E ; Alencar, Bruna CG ; Bargieri, Daniel Y ; Boscardin, Silvia B ; Rodrigues, Maurício M ; Ferreira, Luís CS
  • Assuntos: Antigens ; Cytotoxicity ; Genetic engineering ; Immune system ; Lymphocytes ; Peptides ; Plasmids ; Proteins ; Vaccines
  • É parte de: Vaccine, 2010-02, Vol.28 (5), p.1373
  • Descrição: Salmonellaflagellin, the flagellum structural subunit, has received particular interest as a vaccine adjuvant conferring enhanced immunogenity to soluble proteins or peptides, both for activation of antibody and cellular immune responses. In the present study, we evaluated theSalmonella entericaFliCdflagellin as a T cell vaccine adjuvant using as model the 9-mer (SYVPSAEQI) synthetic H2d-restricted CD8+T cell-specific epitope (CS280-288) derived from thePlasmodium yoeliicircumsporozoite (CS) protein. The FliCdadjuvant effects were determined under two different conditions: (i) as recombinant flagella, expressed by orally delivered liveS. Dublinvaccine strains expressing the target CS280-288peptide fused at the central hypervariable domain, and (ii) as purified protein in acellular vaccines in which flagellin was administered to mice either as a recombinant protein fused or admixed with the target CS280-288peptide. The results showed that CS280-288-specific cytotoxic CD8+T cells were primed when BALB/c mice were orally inoculated with the expressing the CS280-288epitopeS. Dublinvaccine strain. In contrast, mice immunized with purified FliCdadmixed with the CS280-288peptide and, to a lesser extent, fused with the target peptide developed specific cytotoxic CD8+T cell responses without the need of a heterologous booster immunization. The CD8+T cell adjuvant effects of flagellin, either fused or not with the target peptide, correlated with the in vivo activation of CD11c+dendritic cells. Taken together, the present results demonstrate thatSalmonellaflagellins are flexible adjuvant and induce adaptative immune responses when administered by different routes or vaccine formulations.
  • Editor: Kidlington: Elsevier Limited
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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