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Microporous dermal-mimetic electrospun scaffolds pre-seeded with fibroblasts promote tissue regeneration in full-thickness skin wounds

Bonvallet, Paul P ; Schultz, Matthew J ; Mitchell, Elizabeth H ; Bain, Jennifer L ; Culpepper, Bonnie K ; Thomas, Steven J ; Bellis, Susan L Engler, Adam J.

PloS one, 2015-03, Vol.10 (3), p.e0122359-e0122359 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Microporous dermal-mimetic electrospun scaffolds pre-seeded with fibroblasts promote tissue regeneration in full-thickness skin wounds
Autor: Bonvallet, Paul P ; Schultz, Matthew J ; Mitchell, Elizabeth H ; Bain, Jennifer L ; Culpepper, Bonnie K ; Thomas, Steven J ; Bellis, Susan L
Engler, Adam J.
Assuntos: Animals ; Biology ; Biomedical engineering ; Biomedical materials ; Biomimetic Materials - pharmacology ; Caprolactone ; Care and treatment ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Chemical composition ; Collagen ; Collagen (type I) ; Collagen - metabolism ; Collagens ; Comparative analysis ; Contraction ; Defects ; Dermis - drug effects ; Dermis - pathology ; Electrospinning ; Extracellular matrix ; Extracellular Matrix - drug effects ; Extracellular Matrix - metabolism ; Fibroblasts ; Fibroblasts - cytology ; Fibronectin ; Fibronectins ; Fibronectins - metabolism ; Healing ; Implantation ; Infiltration ; Mechanical properties ; Plastic surgery ; Polyesters - pharmacology ; Pores ; Porosity ; Proteins ; Rats, Inbred F344 ; Regeneration ; Regeneration - drug effects ; Scaffolds ; Seeding ; Skin ; Skin & tissue grafts ; Stem cells ; Studies ; Substrates ; Surgical implants ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Transplants & implants ; Wound care ; Wound healing ; Wound Healing - drug effects ; Wounds and injuries
É parte de: PloS one, 2015-03, Vol.10 (3), p.e0122359-e0122359
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: PPB SJT SLB. Performed the experiments: PPB MJS EHM JLB BKC. Analyzed the data: PPB EHM SLB. Contributed reagents/materials/analysis tools: EHM. Wrote the paper: PPB SLB.
Descrição: Electrospun scaffolds serve as promising substrates for tissue repair due to their nanofibrous architecture and amenability to tailoring of chemical composition. In this study, the regenerative potential of a microporous electrospun scaffold pre-seeded with dermal fibroblasts was evaluated. Previously we reported that a 70% collagen I and 30% poly(Ɛ-caprolactone) electrospun scaffold (70:30 col/PCL) containing 160 μm diameter pores had favorable mechanical properties, supported fibroblast infiltration and subsequent cell-mediated deposition of extracellular matrix (ECM), and promoted more rapid and effective in vivo skin regeneration when compared to scaffolds lacking micropores. In the current study we tested the hypothesis that the efficacy of the 70:30 col/PCL microporous scaffolds could be further enhanced by seeding scaffolds with dermal fibroblasts prior to implantation into skin wounds. To address this hypothesis, a Fischer 344 (F344) rat syngeneic model was employed. In vitro studies showed that dermal fibroblasts isolated from F344 rat skin were able to adhere and proliferate on 70:30 col/PCL microporous scaffolds, and the cells also filled the 160 μm pores with native ECM proteins such as collagen I and fibronectin. Additionally, scaffolds seeded with F344 fibroblasts exhibited a low rate of contraction (~14%) over a 21 day time frame. To assess regenerative potential, scaffolds with or without seeded F344 dermal fibroblasts were implanted into full thickness, critical size defects created in F344 hosts. Specifically, we compared: microporous scaffolds containing fibroblasts seeded for 4 days; scaffolds containing fibroblasts seeded for only 1 day; acellular microporous scaffolds; and a sham wound (no scaffold). Scaffolds containing fibroblasts seeded for 4 days had the best response of all treatment groups with respect to accelerated wound healing, a more normal-appearing dermal matrix structure, and hair follicle regeneration. Collectively these results suggest that microporous electrospun scaffolds pre-seeded with fibroblasts promote greater wound-healing than acellular scaffolds.
Editor: United States: Public Library of Science
Idioma: Inglês

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Microporous dermal-mimetic electrospun scaffolds pre-seeded with fibroblasts promote tissue regeneration in full-thickness skin wounds

Bonvallet, Paul P ; Schultz, Matthew J ; Mitchell, Elizabeth H ; Bain, Jennifer L ; Culpepper, Bonnie K ; Thomas, Steven J ; Bellis, Susan L Engler, Adam J.

United States: Public Library of Science

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