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Different migratory patterns of blood, splenic, thymic lymphocytes, T and NK cells labeled with In-111 tropolone in rats

Gagne, G. ; Garcia, A.M. ; McAfee, J.G.

J. Nucl. Med.; (United States), 1984, Vol.25:5

United States

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Título:
Different migratory patterns of blood, splenic, thymic lymphocytes, T and NK cells labeled with In-111 tropolone in rats
Autor: Gagne, G. ; Garcia, A.M. ; McAfee, J.G.
Assuntos: ANIMAL CELLS ; ANIMAL TISSUES ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BIOLOGICAL MATERIALS ; BLOOD ; BLOOD CELLS ; BODY ; BODY FLUIDS ; BONE MARROW ; CONNECTIVE TISSUE CELLS ; DAYS LIVING RADIOISOTOPES ; DIGESTIVE SYSTEM ; DISTRIBUTION ; DRUGS ; ELECTRON CAPTURE RADIOISOTOPES ; GLANDS ; HEMATOPOIETIC SYSTEM ; IMMUNOLOGY ; INDIUM 111 ; INDIUM ISOTOPES ; INTERMEDIATE MASS NUCLEI ; ISOMERIC TRANSITION ISOTOPES ; ISOTOPES ; LABELLED COMPOUNDS ; LABELLING ; LEUKOCYTES ; LIVER ; LUNGS ; LYMPH VESSELS ; LYMPHATIC SYSTEM ; LYMPHOCYTES ; MAMMALS ; MATERIALS ; MEDICINE ; MIGRATION ; MINUTES LIVING RADIOISOTOPES ; NUCLEAR MEDICINE ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANS ; RADIOIMMUNOLOGY ; RADIOIMMUNOTHERAPY ; RADIOISOTOPES ; RADIOLOGY ; RADIONUCLIDE KINETICS ; RADIOPHARMACEUTICALS ; RADIOTHERAPY ; RATS ; RESPIRATORY SYSTEM ; RODENTS ; SOMATIC CELLS ; SPLEEN ; THERAPY ; TISSUE DISTRIBUTION ; TISSUES ; VERTEBRATES 551001 -- Physiological Systems-- Tracer Techniques
É parte de: J. Nucl. Med.; (United States), 1984, Vol.25:5
Notas: CONF-840619-
None
Descrição: Labeled cells harvested from different lymphoid organs have been used to study lymphocyte migration in experimental animals, but the influence of the source organ on their biodistribution is largely unknown. The authors harvested peripheral blood cells (88% lymphocytes), splenic cells, splenic T cells after elimination of BETA cells by nylon wool column, thymic cells and natural killer (NK) enriched cells. For the last preparation, spleen cells were obtained from neonatal thymectomized rats, nylon wool column filtered, and centrifuged on Percoll, to obtain 27% NK cells. These 5 different cell preparations were labeled with (In-111 tropolone (<10..mu..Ci/10/sup 8/ cells) and their blood clearance and localization were compared in Wistar rats. Peripheral blood cells maintained a high blood level up to 24 hr. (27%), similar to previous studies in humans. They reached a high concentration in lymph nodes (25%), with comparatively low levels in the spleen and liver. In contrast, cells of splenic or thymic origin disappeared rapidly from the blood and sequestered temporarily in the lungs. Thymic cells were sequestered in the liver, and concentrated poorly in lymph nodes. Splenic T cells had a relatively high concentration in lymph nodes and marrow. NK enriched cells maintained a relatively high level in the blood, a low concentration in lymph nodes, and sequestered chiefly in the liver. These results indicate that the source organ from which lymphocytes are extracted is a major determinant in their localization.
Editor: United States
Idioma: Inglês

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Different migratory patterns of blood, splenic, thymic lymphocytes, T and NK cells labeled with In-111 tropolone in rats

Gagne, G. ; Garcia, A.M. ; McAfee, J.G.

United States

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