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The characterisation of thiobarbituric acid reactivity in human plasma and urine

Gutteridge, J.M.C. ; Tickner, T.R.

Analytical biochemistry, 1978-11, Vol.91 (1), p.250-257 [Periódico revisado por pares]

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Título:
The characterisation of thiobarbituric acid reactivity in human plasma and urine
Autor: Gutteridge, J.M.C. ; Tickner, T.R.
Assuntos: Chromatography, Thin Layer ; Humans ; Lipid Peroxidation ; Lipids - blood ; Lipids - chemistry ; Malondialdehyde - blood ; Malondialdehyde - urine ; Spectrometry, Fluorescence ; Thiobarbituric Acid Reactive Substances - analysis
É parte de: Analytical biochemistry, 1978-11, Vol.91 (1), p.250-257
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The specificity of the thiobarbituric acid reaction (TBA) has been investigated using techniques of high-performance thin-layer chromatography and spectrofluorimetry. It was found that malondialdehyde (MDA) derived from different lipid and nonlipid origins formed the same MDA-TBA complex. This complex could be separated from other TBA-reactive compounds by both chromatography and spectrofluorimetry. Normal human plasma and urine both formed an MDA-TBA complex along with other TBA-reactive compounds. In plasma this was associated mainly with phosphatidylcholine and appeared to be peroxidic in reaction. Urine, however, contained polar MDA-forming compounds probably resulting from the oxidation of 2-deoxyaldoses during the acid-heating stage of the TBA test.
Editor: United States: Elsevier Inc
Idioma: Inglês

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