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Hypoxia Does Not Alter Angiotensin Converting Enzyme Activity in Hamster Pulmonary Microvessels

Shepard, Jonathan M ; Joyner, William L ; Gilmore, Joseph P

Circulation research, 1987-08, Vol.61 (2), p.228-235 [Periódico revisado por pares]

Hagerstown, MD: American Heart Association, Inc

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Título:
Hypoxia Does Not Alter Angiotensin Converting Enzyme Activity in Hamster Pulmonary Microvessels
Autor: Shepard, Jonathan M ; Joyner, William L ; Gilmore, Joseph P
Assuntos: Angiotensin I - metabolism ; Angiotensin I - pharmacology ; Angiotensin II - metabolism ; Angiotensin II - pharmacology ; Animals ; Arteries - enzymology ; Arterioles - enzymology ; Biological and medical sciences ; Cardiology. Vascular system ; Cheek - blood supply ; Chronic cor pulmonale ; Cricetinae ; Female ; Heart ; Hypoxia - enzymology ; Hypoxia - physiopathology ; Lung - blood supply ; Lung Transplantation ; Medical sciences ; Mesocricetus ; Methods ; Microcirculation - enzymology ; Peptidyl-Dipeptidase A - analysis ; Peptidyl-Dipeptidase A - metabolism ; Pneumology ; Pulmonary Circulation ; Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases ; Veins - enzymology ; Venules - enzymology
É parte de: Circulation research, 1987-08, Vol.61 (2), p.228-235
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Studies were initiated to investigate the effects of hypoxia on the conversion of angiotensin I (AI) to angiotensin II (AII) in microvessels of the lung. Using the technique of allografting neonatal lung tissue into the cheek pouch of normal hamsters, the microvessels of the lung, pulmonary arterioles, and venules could be visualized and manipulated by direct in vivo microscopy. The microvessels of the lung were studied 7–10 days after allografting by anesthetizing the hamster with pentobarbital (6.0 mg/100 g body weight i.p.) and then preparing the lung tissue for observation. The tissue was suffused with a Ringerʼs bicarbonate solution bubbled with a normal (20% O2-5% CO2-75% N2 or a low (95% N2-5% CO2) oxygen mixture. After equilibration, a pulmonary arteriole or venule was selected for observation, and the vessel geometry was recorded. Then, a micropipette containing either AI or AII was positioned alongside the vessel, and the agent was delivered continuously for 2 minutes. Lumen diameter was recorded continually for 8–10 minutes. This procedure was repeated until both angiotensins were tested on pulmonary arterioles and venules under conditions of a normal and low oxygen environment. This protocol was repeated on cheek pouch microvessels that did not contain pulmonary allografts. Both AI and AII produced rapid decreases in the lumen diameters of all microvessels tested. This vasoconstriction was greater for AII, and the oxygen environment did not alter the response. Conversion of AI to AII was not altered by the oxygen environment, and the relative conversion was similar in the microvessels of the lung and cheek pouch. These studies demonstrated that acute hypoxia does not alter the conversion of angiotensin in either pulmonary or cheek pouch microvessels. Thus, an altered enzymatic activity may be restricted by the endothelial cell surface area available for conversion of angiotensin.
Editor: Hagerstown, MD: American Heart Association, Inc
Idioma: Inglês

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Hypoxia Does Not Alter Angiotensin Converting Enzyme Activity in Hamster Pulmonary Microvessels

Shepard, Jonathan M ; Joyner, William L ; Gilmore, Joseph P

Hagerstown, MD: American Heart Association, Inc

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