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Specific Coordination between Zr-MOF and Phosphate-Terminated DNA Coupled with Strand Displacement for the Construction of Reusable and Ultrasensitive Aptasensor

Qiu, Weiwei ; Gao, Feng ; Yano, Natsumi ; Kataoka, Yusuke ; Handa, Makoto ; Yang, Weiqiang ; Tanaka, Hidekazu ; Wang, Qingxiang

Analytical chemistry (Washington), 2020-08, Vol.92 (16), p.11332-11340 [Periódico revisado por pares]

United States: American Chemical Society

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Título:
Specific Coordination between Zr-MOF and Phosphate-Terminated DNA Coupled with Strand Displacement for the Construction of Reusable and Ultrasensitive Aptasensor
Autor: Qiu, Weiwei ; Gao, Feng ; Yano, Natsumi ; Kataoka, Yusuke ; Handa, Makoto ; Yang, Weiqiang ; Tanaka, Hidekazu ; Wang, Qingxiang
Assuntos: Aptamers ; Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - genetics ; Biosensing Techniques - methods ; Coordination ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA probes ; Electrochemical Techniques ; Electrochemistry ; Food Contamination - analysis ; Food safety ; Immobilized Nucleic Acids - chemistry ; Immobilized Nucleic Acids - genetics ; Limit of Detection ; Metal-organic frameworks ; Metal-Organic Frameworks - chemistry ; Methylene blue ; Methylene Blue - chemistry ; Nanoparticles - chemistry ; Nucleic Acid Hybridization ; Ochratoxin A ; Ochratoxins - analysis ; Ochratoxins - chemistry ; Organometallic Compounds - chemistry ; Oxidation-Reduction ; Phosphates - chemistry ; Phthalic Acids - chemistry ; Sensitivity enhancement ; Wine ; Wine - analysis ; Zirconium ; Zirconium - chemistry
É parte de: Analytical chemistry (Washington), 2020-08, Vol.92 (16), p.11332-11340
Descrição: Electrochemical aptasensors involved in chemical labeling are often single-use and sensitivity-limited because the probes are commonly single-point labeled and irreversible. In this work, the specific coordination between Zr4+ and phosphate group (−PO4 3–) was employed to construct a new aptasensor that is highly sensitive and reusable, using Ochratoxin A (OTA) as the test model. The OTA binding aptamer (OBA) was hybridized with the thiolated supporting sequence (TSS) immobilized on the surface of a gold electrode. The UiO-66 with a formula of [Zr6O4(OH)4(BDC)6], one of the class of Zr metal–organic frameworks (MOFs), was then particularly grafted on the terminal of OBA through the specific coordination between Zr4+ and 5′-PO4 3–, i.e., the Zr–O–P coordination bond. Similarly, as much as the 5′-PO4 3– and 3′-methylene blue dual-labeled sequences (DLS) were further assembled on UiO-66 due to the large surface area of MOF and rich active sites of Zr4+. Owing to the specific coordination for signal amplification, the developed aptasensor shows greatly enhanced sensitivity. A wide detection range from 0.1 fM to 2.0 μM and an ultralow detection limit of 0.079 fM (S/N = 3) for OTA were obtained. Additionally, the TSS can rehybridize with a new OBA to regenerate the aptasensor but without complicated pretreatments, enabling a aptasensor that is readily reusable for OTA detection. The aptasensor was successfully applied for OTA detection in the red wine samples, demonstrating a promising prospect for food safety monitoring.
Editor: United States: American Chemical Society
Idioma: Inglês

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Specific Coordination between Zr-MOF and Phosphate-Terminated DNA Coupled with Strand Displacement for the Construction of Reusable and Ultrasensitive Aptasensor

Qiu, Weiwei ; Gao, Feng ; Yano, Natsumi ; Kataoka, Yusuke ; Handa, Makoto ; Yang, Weiqiang ; Tanaka, Hidekazu ; Wang, Qingxiang

United States: American Chemical Society

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