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Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice

Galli, Emilia ; Rossi, Jari ; Neumann, Thomas ; Andressoo, Jaan-Olle ; Drinda, Stefan ; Lindholm, Päivi

Scientific reports, 2019-10, Vol.9 (1), p.14318-12, Article 14318 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice
Autor: Galli, Emilia ; Rossi, Jari ; Neumann, Thomas ; Andressoo, Jaan-Olle ; Drinda, Stefan ; Lindholm, Päivi
Assuntos: Adiponectin ; Adiponectin - blood ; Adult ; Age ; Aged ; Animals ; Cell culture ; Degenerative diseases ; Diet, High-Fat - adverse effects ; Dietary restrictions ; Endoplasmic reticulum ; Enzyme-linked immunosorbent assay ; Fasting - blood ; Fasting - metabolism ; High fat diet ; Homeostasis ; Humans ; Liver ; Liver - chemistry ; Liver - metabolism ; Medicin och hälsovetenskap ; Menopause ; Mesencephalon - metabolism ; Metabolism ; Mice ; Mice, Knockout ; Mice, Obese ; Middle Aged ; Nerve Growth Factors - blood ; Nerve Growth Factors - genetics ; Nerve Growth Factors - metabolism ; Neurotrophic factors ; Protein folding ; Up-Regulation ; Young Adult
É parte de: Scientific reports, 2019-10, Vol.9 (1), p.14318-12, Article 14318
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Dietary restriction induces beneficial metabolic changes and prevents age-related deterioration. Mesencephalic astrocyte-derived neurotrophic factor (MANF) shows protective effects on cells in various models of degenerative diseases. Here we studied whether circulating concentrations of MANF are associated with fasting-induced positive effects. We quantified the levels of circulating MANF from 40 human subjects before and after therapeutic fasting. As measured by an enzyme-linked immunosorbent assay (ELISA), the mean concentration of plasma MANF increased after an average fasting of 15 days. Plasma MANF levels correlated inversely with adiponectin, a hormone that regulates metabolism, thus suggesting that MANF levels are related to metabolic homeostasis. To study the effects of dietary intervention on MANF concentrations in mice, we developed an ELISA for mouse MANF and verified its specificity using MANF knock-out (KO) tissue. A switch from high-fat to normal diet increased MANF levels and downregulated the expression of unfolded protein response (UPR) genes in the liver, indicating decreased endoplasmic reticulum (ER) stress. Liver MANF and serum adiponectin concentrations correlated inversely in mice. Our findings demonstrate that MANF expression and secretion increases with dietary intervention. The MANF correlation to adiponectin and its possible involvement in metabolic regulation and overall health warrants further studies.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice

Galli, Emilia ; Rossi, Jari ; Neumann, Thomas ; Andressoo, Jaan-Olle ; Drinda, Stefan ; Lindholm, Päivi

England: Nature Publishing Group

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