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Transforming Growth Factor β1-regulated Xylosyltransferase I Activity in Human Cardiac Fibroblasts and Its Impact for Myocardial Remodeling

Prante, Christian ; Milting, Hendrik ; Kassner, Astrid ; Farr, Martin ; Ambrosius, Michael ; Schön, Sylvia ; Seidler, Daniela G. ; Banayosy, Aly El ; Körfer, Reiner ; Kuhn, Joachim ; Kleesiek, Knut ; Götting, Christian

The Journal of biological chemistry, 2007-09, Vol.282 (36), p.26441-26449 [Periódico revisado por pares]

Elsevier Inc

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  • Título:
    Transforming Growth Factor β1-regulated Xylosyltransferase I Activity in Human Cardiac Fibroblasts and Its Impact for Myocardial Remodeling
  • Autor: Prante, Christian ; Milting, Hendrik ; Kassner, Astrid ; Farr, Martin ; Ambrosius, Michael ; Schön, Sylvia ; Seidler, Daniela G. ; Banayosy, Aly El ; Körfer, Reiner ; Kuhn, Joachim ; Kleesiek, Knut ; Götting, Christian
  • É parte de: The Journal of biological chemistry, 2007-09, Vol.282 (36), p.26441-26449
  • Descrição: In cardiac fibrosis remodeling of the failing myocardium is associated with a complex reorganization of the extracellular matrix (ECM). Xylosyltransferase I and Xylosyltransferase II (XT-I and XT-II) are the key enzymes in proteoglycan biosynthesis, which are an important fraction of the ECM. XT-I was shown to be a measure for the proteoglycan biosynthesis rate and a biochemical fibrosis marker. Here, we investigated the XT-I and XT-II expression in cardiac fibroblasts and in patients with dilated cardiomyopathy and compared our findings with nonfailing donor hearts. We analyzed XT-I and XT-II expression and the glycosaminoglycan (GAG) content in human cardiac fibroblasts incubated with transforming growth factor (TGF)-β1 or exposed to cyclic mechanical stretch. In vitro and in vivo no significant changes in the XT-II expression were detected. For XT-I we found an increased expression in parallel with an elevated chondroitin sulfate-GAG content after incubation with TGF-β1 and after mechanical stretch. XT-I expression and subsequently increased levels of GAGs could be reduced with neutralizing anti-TGF-β1 antibodies or by specific inhibition of the activin receptor-like kinase 5 or the p38 mitogen-activated protein kinase pathway. Usage of XT-I small interfering RNA could specifically block the increased XT-I expression under mechanical stress and resulted in a significantly reduced chondroitin sulfate-GAG content. In the left and right ventricular samples of dilated cardiomyopathy patients, our data show increased amounts of XT-I mRNA compared with nonfailing controls. Patients had raised levels of XT-I enzyme activity and an elevated proteoglycan content. Myocardial remodeling is characterized by increased XT-I expression and enhanced proteoglycan deposition. TGF-β1 and mechanical stress induce XT-I expression in cardiac fibroblasts and have impact for ECM remodeling in the dilated heart. Specific blocking of XT-I expression confirmed that XT-I catalyzes a rate-limiting step during fibrotic GAG biosynthesis.
  • Editor: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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