Idioma:

Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses

Antonucci, Flavia ; Corradini, Irene ; Morini, Raffaella ; Fossati, Giuliana ; Menna, Elisabetta ; Pozzi, Davide ; Pacioni, Simone ; Verderio, Claudia ; Bacci, Alberto ; Matteoli, Michela

EMBO reports, 2013-07, Vol.14 (7), p.645-651 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses
Autor: Antonucci, Flavia ; Corradini, Irene ; Morini, Raffaella ; Fossati, Giuliana ; Menna, Elisabetta ; Pozzi, Davide ; Pacioni, Simone ; Verderio, Claudia ; Bacci, Alberto ; Matteoli, Michela
Assuntos: Action Potentials - drug effects ; Action Potentials - physiology ; Animals ; Calcium - metabolism ; Calcium Channels - genetics ; Calcium Channels - metabolism ; gamma-Aminobutyric Acid - metabolism ; gamma-Aminobutyric Acid - pharmacology ; Gene Expression Regulation, Developmental ; Gene Silencing ; Genotype & phenotype ; glutamatergic transmission ; Glutamic Acid - metabolism ; Glutamic Acid - pharmacology ; Hippocampus - cytology ; Hippocampus - drug effects ; Humans ; Mice ; Neurology ; Neuronal Plasticity - drug effects ; Neuronal Plasticity - physiology ; Neurons - cytology ; Neurons - drug effects ; Neurons - physiology ; Neuropsychology ; Neurotransmitters ; Primary Cell Culture ; Psychiatry ; Rats ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Scientific Reports ; short-term plasticity ; SNAP-25 ; Synaptic Transmission - drug effects ; Synaptic Transmission - physiology ; Synaptic Vesicles - drug effects ; Synaptic Vesicles - physiology ; Synaptosomal-Associated Protein 25 - antagonists & inhibitors ; Synaptosomal-Associated Protein 25 - genetics ; Synaptosomal-Associated Protein 25 - metabolism
É parte de: EMBO reports, 2013-07, Vol.14 (7), p.645-651
Notas: Supplementary InformationReview Process File
istex:65C36583C79974E42C172458B7F6114B89048BCA
ark:/67375/WNG-WVX41XLV-J
ArticleID:EMBR201375
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: SNAP‐25 is a key component of the synaptic‐vesicle fusion machinery, involved in several psychiatric diseases including schizophrenia and ADHD. SNAP‐25 protein expression is lower in different brain areas of schizophrenic patients and in ADHD mouse models. How the reduced expression of SNAP‐25 alters the properties of synaptic transmission, leading to a pathological phenotype, is unknown. We show that, unexpectedly, halved SNAP‐25 levels at 13–14 DIV not only fail to impair synaptic transmission but instead enhance evoked glutamatergic neurotransmission. This effect is possibly dependent on presynaptic voltage‐gated calcium channel activity and is not accompanied by changes in spontaneous quantal events or in the pool of readily releasable synaptic vesicles. Notably, synapses of 13–14 DIV neurons with reduced SNAP‐25 expression show paired‐pulse depression as opposed to paired‐pulse facilitation occurring in their wild‐type counterparts. This phenotype disappears with synapse maturation. As alterations in short‐term plasticity represent a new mechanism contributing to cognitive impairments in intellectual disabilities, our data provide mechanistic clues for neuronal circuit alterations in psychiatric diseases characterized by reduced expression of SNAP‐25. SNAP‐25, a key component of the synapse, is reduced in brain areas of schizophrenic patients. This study shows that SNAP‐25 levels alter short‐term plasticity, which might contribute to neural alterations in psychiatric diseases.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses

Antonucci, Flavia ; Corradini, Irene ; Morini, Raffaella ; Fossati, Giuliana ; Menna, Elisabetta ; Pozzi, Davide ; Pacioni, Simone ; Verderio, Claudia ; Bacci, Alberto ; Matteoli, Michela

Chichester, UK: John Wiley & Sons, Ltd

Buscando em bases de dados remotas. Favor aguardar.