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Monitoring endangered freshwater biodiversity using environmental DNA

THOMSEN, PHILIP FRANCIS ; KIELGAST, JOS ; IVERSEN, LARS L. ; WIUF, CARSTEN ; RASMUSSEN, MORTEN ; GILBERT, M. THOMAS P ; ORLANDO, LUDOVIC ; WILLERSLEV, ESKE

Molecular ecology, 2012-06, Vol.21 (11), p.2565-2573 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Monitoring endangered freshwater biodiversity using environmental DNA
Autor: THOMSEN, PHILIP FRANCIS ; KIELGAST, JOS ; IVERSEN, LARS L. ; WIUF, CARSTEN ; RASMUSSEN, MORTEN ; GILBERT, M. THOMAS P ; ORLANDO, LUDOVIC ; WILLERSLEV, ESKE
Assuntos: Amphibians - genetics ; Animals ; Biodiversity ; biological diversity ; Conservation of Natural Resources ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; Endangered Species ; Environmental Monitoring - methods ; Environmental studies ; Europe ; Fishes - genetics ; Fresh Water ; Freshwater ecology ; Insecta - genetics ; Lakes ; molecular detection ; pyrosequencing ; threatened species ; Triturus - genetics ; wildlife conservation
É parte de: Molecular ecology, 2012-06, Vol.21 (11), p.2565-2573
Notas: istex:3C538FC212F4D07073056BD820476EE3E7734370
ArticleID:MEC5418
ark:/67375/WNG-ZPC7GJJQ-B
These authors contributed equally to this study.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Freshwater ecosystems are among the most endangered habitats on Earth, with thousands of animal species known to be threatened or already extinct. Reliable monitoring of threatened organisms is crucial for data‐driven conservation actions but remains a challenge owing to nonstandardized methods that depend on practical and taxonomic expertise, which is rapidly declining. Here, we show that a diversity of rare and threatened freshwater animals—representing amphibians, fish, mammals, insects and crustaceans—can be detected and quantified based on DNA obtained directly from small water samples of lakes, ponds and streams. We successfully validate our findings in a controlled mesocosm experiment and show that DNA becomes undetectable within 2 weeks after removal of animals, indicating that DNA traces are near contemporary with presence of the species. We further demonstrate that entire faunas of amphibians and fish can be detected by high‐throughput sequencing of DNA extracted from pond water. Our findings underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for monitoring rare and threatened species across a wide range of taxonomic groups. See also the Perspective by Lodge et al
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Monitoring endangered freshwater biodiversity using environmental DNA

THOMSEN, PHILIP FRANCIS ; KIELGAST, JOS ; IVERSEN, LARS L. ; WIUF, CARSTEN ; RASMUSSEN, MORTEN ; GILBERT, M. THOMAS P ; ORLANDO, LUDOVIC ; WILLERSLEV, ESKE

Oxford, UK: Blackwell Publishing Ltd

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