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Process optimization for enzymatic production of a valuable biomass-based ester from levulinic acid

Marcus V. S. Cambraia Milson S Barbosa; Cleide Mara Faria Soares; Ana Karine Furtado de Carvalho; Adriano Aguiar Mendes

Bioprocess and biosystems engineering v.46, p.53-67, 2023

Springer 2023

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  • Título:
    Process optimization for enzymatic production of a valuable biomass-based ester from levulinic acid
  • Autor: Marcus V. S. Cambraia
  • Milson S Barbosa; Cleide Mara Faria Soares; Ana Karine Furtado de Carvalho; Adriano Aguiar Mendes
  • Assuntos: BIOMASSA; LIPASE; Eversa® Transform 2.0; Lipase Immobilization; Biomass-Based Materials; Levulinic Acid Alkyl Ester; Optimization
  • É parte de: Bioprocess and biosystems engineering v.46, p.53-67, 2023
  • Notas: Disponível em: https://doi.org/10.1007/s00449-022-02813-w. Acesso em: 26 out. 2023
  • Descrição: The enzymatic production of isoamyl levulinate via esterification of isoamyl alcohol (IA) and levulinic acid (LA), a biomass-based platform chemical with attractive properties, in a solvent system has been performed in this study. For such a purpose, a low-cost liquid lipase (Eversa® Transform 2.0) immobilized by physical adsorption via hydrophobic interactions (mechanism of interfacial activation) on mesoporous poly(styrenene-divinylbenzene) (PSty-DVB) beads was used as heterogeneous biocatalyst. It was prepared at low ionic strength (5 mmol.L−1 buffer sodium acetate pH 5.0) and 25 ℃ using an initial protein loading of 40 mg.g−1 of support. Maximum protein loading of 31.2 ± 2.8 mg.g−1 of support and an immobilization yield of 83% was achieved. The influence of relevant factors (biocatalyst concentration and reaction temperature) on ester production was investigated using a central composite rotatable design (CCRD). Maximum acid conversion percentage of 65% was achieved after 12 h of reaction at 40 °C, 20% of mass of heterogeneous biocatalyst per mass of reaction mixture (20% m.m−1), and LA:IA molar ratio of 1:1.5 in a methyl isobutyl ketone (MIBK) medium. The biocatalyst retained around of 30% of its initial activity after five consecutive esterification batches under optimal experimental conditions. The proposed experimental procedure can be considered as an acceptable green process (EcoScale score of 66.5), in addition to the fact that a new strategy is proposed to sustainably produce a valuable industrial ester (isoamyl levulinate) from biomass-based materials using an immobilized and low-cost commercial lipase as catalyst.
  • Editor: Springer
  • Data de criação/publicação: 2023
  • Formato: p. 53-67.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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