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The DA6-147 monoclonal antibody raised against the HLA-DRα chain identifies a cryptic epitope on the BoLA-DRα chain

ABABOU, A ; DAVIS, W. C ; LEVY, D

Veterinary research (Paris), 1993, Vol.24 (5), p.402-407 [Periódico revisado por pares]

Les Ulis: EDP Sciences

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Título:
The DA6-147 monoclonal antibody raised against the HLA-DRα chain identifies a cryptic epitope on the BoLA-DRα chain
Autor: ABABOU, A ; DAVIS, W. C ; LEVY, D
Assuntos: Animal biology ; Animal genetics ; Animals ; Antibodies, Monoclonal - immunology ; Antigens ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Blotting, Western ; Cattle ; Cattle Diseases - pathology ; Cell Behavior ; Cellular Biology ; Epitopes - analysis ; Epitopes - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Genetics ; Histocompatibility antigens (hla, h-2 and other systems) ; Histocompatibility Antigens Class II - chemistry ; Histocompatibility Antigens Class II - immunology ; HLA-DR Antigens - immunology ; Immunology ; Life Sciences ; Lymphoma, Non-Hodgkin - pathology ; Lymphoma, Non-Hodgkin - veterinary ; Microbiology and Parasitology ; Molecular biology ; Molecular immunology ; Neurons and Cognition ; Precipitin Tests ; Santé publique et épidémiologie ; Tumor Cells, Cultured
É parte de: Veterinary research (Paris), 1993, Vol.24 (5), p.402-407
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: By combining immunoprecipitation of BoLA molecules with monoclonal antibodies raised against 2 different major histocompatibility class II antigens, TH14B and TH81A, and western blotting using the anti-HLA DR alpha monoclonal antibody DA6-147, we characterized an epitope conserved on BoLA- and HLA-DR alpha chains. This epitope, not accessible on intact cells, was revealed after cell lysis. In addition, these results allowed us to define TH14B as an anti-BoLA-DR monoclonal antibody whereas TH81A, raised against the products of a second MHC locus, is probably an anti-BoLA-DQ monoclonal antibody.
Editor: Les Ulis: EDP Sciences
Idioma: Inglês;Francês

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The DA6-147 monoclonal antibody raised against the HLA-DRα chain identifies a cryptic epitope on the BoLA-DRα chain

ABABOU, A ; DAVIS, W. C ; LEVY, D

Les Ulis: EDP Sciences

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