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Comparison of GeneXpert MRSA/SA ETA assay with semi-quantitative and quantitative cultures and nuc gene-based qPCR for detection of Staphylococcus aureus in endotracheal aspirate samples

Coppens, Jasmine ; Van Heirstraeten, Liesbet ; Ruzin, Alexey ; Yu, Li ; Timbermont, Leen ; Lammens, Christine ; Matheeussen, Veerle ; McCarthy, Michael ; Jorens, Philippe ; Ieven, Margareta ; Kumar-Singh, Samir ; Goossens, Herman ; Malhotra-Kumar, Surbhi

Antimicrobial resistance & infection control, 2019-01, Vol.8 (1), p.4-4, Article 4 [Periódico revisado por pares]

England: BioMed Central Ltd

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Comparison of GeneXpert MRSA/SA ETA assay with semi-quantitative and quantitative cultures and nuc gene-based qPCR for detection of Staphylococcus aureus in endotracheal aspirate samples
Autor: Coppens, Jasmine ; Van Heirstraeten, Liesbet ; Ruzin, Alexey ; Yu, Li ; Timbermont, Leen ; Lammens, Christine ; Matheeussen, Veerle ; McCarthy, Michael ; Jorens, Philippe ; Ieven, Margareta ; Kumar-Singh, Samir ; Goossens, Herman ; Malhotra-Kumar, Surbhi
Assuntos: Analysis ; Antibacterial agents ; Bacterial Proteins - genetics ; Cepheid ; COLOREX™ staph aureus ; Diagnostic tests ; Diagnostic Tests, Routine - methods ; DNA ; Genetic research ; Health aspects ; Humans ; Infection ; Intensive care ; Internet ; Laboratories ; Methicillin ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - growth & development ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbiology ; Micrococcal Nuclease - genetics ; Nosocomial infections ; Pathogens ; Pneumonia ; Pneumonia, Ventilator-Associated ; Rapid diagnostics ; Real-time PCR ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics ; Staphylococcus aureus - growth & development ; Staphylococcus aureus - isolation & purification ; Staphylococcus infections ; Surveillance ; Systematic review ; VAP ; Ventilator-associated pneumonia ; Ventilators ; Ventilators, Mechanical - microbiology
É parte de: Antimicrobial resistance & infection control, 2019-01, Vol.8 (1), p.4-4, Article 4
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: is a common cause of ventilator-associated pneumonia. Rapid and accurate detection of lower respiratory tract colonization and/or infection with may inform targeted preventive and therapeutic strategies. To investigate this, we compared semi-quantitative (SQ)-culture results from 79 endotracheal aspirates (ETA) collected from mechanically-ventilated patients, to two culture and two non-culture-based methods for detection of . ETA analyzed by routine SQ-culture on blood and colistin-nalidixic-acid agar was compared to: (i) quantitative (Q-) culture on chromogenic COLOREX™ Staph aureus; (ii) enrichment in brain-heart-infusion broth followed by plating on blood agar and COLOREX™; (iii) -based TaqMan qPCR, and (iv) GeneXpert MRSA/SA ETA assay. Of the 79 ETA samples analyzed by SQ-culture, 39 samples were positive, and 40 negative for . Two samples negative for by SQ-culture were, however, -positive by the other four methods and were considered positive. Appending these two samples as positive in the SQ-culture results, sensitivities-specificities for Q-culture, enrichment-culture, TaqMan qPCR and GeneXpert were 100-95, 100-92, 100-53% and 100% - 100, respectively. The lower specificities of Q-culture, enrichment-culture, and TaqMan qPCR was because of their higher sensitivities, although TaqMan qPCR also detected -specific extracellular DNA. This first evaluation of the GeneXpert MRSA/SA ETA assay with ETA samples found it to be highly sensitive, specific, user-friendly (hands-on time ~ 5 min.), and rapid (~ 66 min. assay time). Where this equipment is not available, we recommend implementing more sensitive culture-based methods for improved detection in ETA samples.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Comparison of GeneXpert MRSA/SA ETA assay with semi-quantitative and quantitative cultures and nuc gene-based qPCR for detection of Staphylococcus aureus in endotracheal aspirate samples

Coppens, Jasmine ; Van Heirstraeten, Liesbet ; Ruzin, Alexey ; Yu, Li ; Timbermont, Leen ; Lammens, Christine ; Matheeussen, Veerle ; McCarthy, Michael ; Jorens, Philippe ; Ieven, Margareta ; Kumar-Singh, Samir ; Goossens, Herman ; Malhotra-Kumar, Surbhi

England: BioMed Central Ltd

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