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Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms

Palacin, A. ; Rodriguez, J. ; Blanco, C. ; Lopez-Torrejon, G. ; Sánchez-Monge, R. ; Varela, J. ; Jiménez, M. A. ; Cumplido, J. ; Carrillo, T. ; Crespo, J. F. ; Salcedo, G.

Clinical and experimental allergy, 2008-07, Vol.38 (7), p.1220-1228 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms
Autor: Palacin, A. ; Rodriguez, J. ; Blanco, C. ; Lopez-Torrejon, G. ; Sánchez-Monge, R. ; Varela, J. ; Jiménez, M. A. ; Cumplido, J. ; Carrillo, T. ; Crespo, J. F. ; Salcedo, G.
Assuntos: Actinidia - immunology ; Actinidia deliciosa ; Adolescent ; Adult ; Allergens - immunology ; Allergens - isolation & purification ; Anaphylaxis - immunology ; Biological and medical sciences ; Child ; Chitinases - immunology ; Chitinases - isolation & purification ; cross-reactive carbohydrate determinant ; Cysteine Endopeptidases - immunology ; Cysteine Endopeptidases - isolation & purification ; Double-Blind Method ; Female ; Food Hypersensitivity - diagnosis ; Food Hypersensitivity - immunology ; Fruit - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins - immunology ; Glycoproteins - isolation & purification ; Humans ; Immunoglobulin E - blood ; kiwi allergy ; latex-fruit syndrome ; Male ; Medical sciences ; Middle Aged ; Persea americana ; Plant Proteins - immunology ; Plant Proteins - isolation & purification ; purified allergen ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Skin Tests ; specific IgE level
É parte de: Clinical and experimental allergy, 2008-07, Vol.38 (7), p.1220-1228
Notas: ark:/67375/WNG-XT4XJGTT-7
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ArticleID:CEA2927
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Descrição: Summary Background Green kiwifruit allergy is on the rise. However, no surveys testing purified major kiwi allergens have been carried out in a large population, including both kiwi‐sensitized [skin prick test (SPT)‐positive] and truly kiwi‐allergic patients. Objective To isolate major kiwifruit allergens, and to explore their relevance by in vitro and in vivo methods in a large kiwi‐sensitized and ‐allergic population. Methods A large group (n=92) of kiwi‐sensitized patients with different clinical symptoms were selected, and double‐blind, placebo‐controlled, food challenges to kiwi were performed in 52 of them. The three major IgE‐binding proteins from kiwifruit extracts were isolated and characterized by N‐terminal amino acid sequencing and molecular size and glycosylation analysis. The allergenic potency of the three kiwi allergens, and of avocado Pers a 1 as a model allergen associated with the latex‐fruit syndrome, was tested by specific IgE quantitation, immunodetection assays and SPTs. Results The isolated kiwifruit allergens were identified as actinidin Act d 1, glycosylated thaumatin‐like Act d 2 and a novel 40 kDa glycoprotein designated as Act d 3.02. Specific IgE to each of the three allergens was found in over 60% of sera from kiwi‐sensitized patients, and Act d 1 and Act d 2 induced positive SPT responses in over 50% of the tested patients. A significant link between IgE levels to Act d 1 and Act d 3 and anaphylaxis was uncovered. Avocado Pers a 1 showed an in vitro sensitization prevalence of around 45%, but a low in vivo reactivity. Conclusion Act d 1, Act d 2 and Act d 3 are major allergens in the population studied. Severe symptoms after kiwi ingestion are associated with high IgE levels to Act d 1 and Act d 3.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms

Palacin, A. ; Rodriguez, J. ; Blanco, C. ; Lopez-Torrejon, G. ; Sánchez-Monge, R. ; Varela, J. ; Jiménez, M. A. ; Cumplido, J. ; Carrillo, T. ; Crespo, J. F. ; Salcedo, G.

Oxford, UK: Blackwell Publishing Ltd

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