Idioma:

MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development

Akers, Johnny C ; Ramakrishnan, Valya ; Kim, Ryan ; Skog, Johan ; Nakano, Ichiro ; Pingle, Sandeep ; Kalinina, Juliya ; Hua, Wei ; Kesari, Santosh ; Mao, Ying ; Breakefield, Xandra O ; Hochberg, Fred H ; Van Meir, Erwin G ; Carter, Bob S ; Chen, Clark C Chen, Mike

PloS one, 2013-10, Vol.8 (10), p.e78115-e78115 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development
Autor: Akers, Johnny C ; Ramakrishnan, Valya ; Kim, Ryan ; Skog, Johan ; Nakano, Ichiro ; Pingle, Sandeep ; Kalinina, Juliya ; Hua, Wei ; Kesari, Santosh ; Mao, Ying ; Breakefield, Xandra O ; Hochberg, Fred H ; Van Meir, Erwin G ; Carter, Bob S ; Chen, Clark C
Chen, Mike
Assuntos: Biomarkers ; Biomarkers, Tumor - cerebrospinal fluid ; Brain cancer ; Cell Line, Tumor ; Cell lines ; Cerebrospinal fluid ; Cerebrospinal Fluid - metabolism ; Consent ; Electronic filing ; Glioblastoma ; Glioblastoma - genetics ; Glioblastoma cells ; Glioblastoma multiforme ; Glyceraldehyde-3-phosphate dehydrogenase ; Hematology ; Hospitals ; Humans ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Neurosciences ; Neurosurgery ; NMR ; Nuclear magnetic resonance ; Oncology ; Patients ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Ribosomal, 18S - genetics ; rRNA 18S ; Vesicles
É parte de: PloS one, 2013-10, Vol.8 (10), p.e78115-e78115
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: We would like to declare that one of the co-author, Johan Skog, is an employee at Exosome Diagnostics. No other authors have any competing interests. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.
These authors also contributed equally to this work.
Conceived and designed the experiments: JCA VR CCC. Performed the experiments: JCA VR. Analyzed the data: SK SP JK EVM IN RK FH BSC XOB JS. Contributed reagents/materials/analysis tools: SK SP JK EVM IN RK FH BSC XOB JS WH YM. Wrote the manuscript: JCA VR CCC.
Descrição: Glioblastoma cells secrete extra-cellular vesicles (EVs) containing microRNAs (miRNAs). Analysis of these EV miRNAs in the bio-fluids of afflicted patients represents a potential platform for biomarker development. However, the analytic algorithm for quantitative assessment of EV miRNA remains under-developed. Here, we demonstrate that the reference transcripts commonly used for quantitative PCR (including GAPDH, 18S rRNA, and hsa-miR-103) were unreliable for assessing EV miRNA. In this context, we quantitated EV miRNA in absolute terms and normalized this value to the input EV number. Using this method, we examined the abundance of miR-21, a highly over-expressed miRNA in glioblastomas, in EVs. In a panel of glioblastoma cell lines, the cellular levels of miR-21 correlated with EV miR-21 levels (p<0.05), suggesting that glioblastoma cells actively secrete EVs containing miR-21. Consistent with this hypothesis, the CSF EV miR-21 levels of glioblastoma patients (n=13) were, on average, ten-fold higher than levels in EVs isolated from the CSF of non-oncologic patients (n=13, p<0.001). Notably, none of the glioblastoma CSF harbored EV miR-21 level below 0.25 copies per EV in this cohort. Using this cut-off value, we were able to prospectively distinguish CSF derived from glioblastoma and non-oncologic patients in an independent cohort of twenty-nine patients (Sensitivity=87%; Specificity=93%; AUC=0.91, p<0.01). Our results suggest that CSF EV miRNA analysis of miR-21 may serve as a platform for glioblastoma biomarker development.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development

Akers, Johnny C ; Ramakrishnan, Valya ; Kim, Ryan ; Skog, Johan ; Nakano, Ichiro ; Pingle, Sandeep ; Kalinina, Juliya ; Hua, Wei ; Kesari, Santosh ; Mao, Ying ; Breakefield, Xandra O ; Hochberg, Fred H ; Van Meir, Erwin G ; Carter, Bob S ; Chen, Clark C Chen, Mike

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.