skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Development and validation of a simple and noninvasive method for salivary uric acid: potential applications for monitoring the salivary uric acid level in healthy volunteers and gout patients

Zhao, Li-Li; Ma, Qingkun; Xu, Chun-Yan; Zheng, Guanghao; Han, Yu; Qian, Qing; Jin, Wenlong; Min, Jun Zhe

Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)

Universidade de São Paulo. Faculdade de Ciências Farmacêuticas 2023-02-28

Acesso online

  • Título:
    Development and validation of a simple and noninvasive method for salivary uric acid: potential applications for monitoring the salivary uric acid level in healthy volunteers and gout patients
  • Autor: Zhao, Li-Li; Ma, Qingkun; Xu, Chun-Yan; Zheng, Guanghao; Han, Yu; Qian, Qing; Jin, Wenlong; Min, Jun Zhe
  • Assuntos: Noninvasive; Gout Patient; Hyperuricemia; Human Saliva; Uric Acid
  • É parte de: Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
  • Descrição: Serum uric acid (UA) is a traditional biomarker in the clinical diagnosis of gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, in this study, a simple and noninvasive method was developed to detect the UA in human saliva to monitor the gout. An Inertsil ODS-3 column was used for the analysis under the condition of isocratic elution with the mixed solution phosphate buffer (74 mM, pH=2.2): Methanol=98:2 (v:v) and the UV detection at 284 nm. Using salivary UA data from healthy volunteers (HVs) (n=68) and gout patients (GPs) (n=14), we examined the salivary UA difference in their content. The intra-and inter-day accuracy and precision (RSD %) were less than 2.56%, the limit of detection (LOD) of UA was 5.0 ng/mL, the mean recoveries of the corresponding compounds were 102.48%. Saliva levels of UA in HVs and GPs were 35.26±14.06 µg/mL and 91.96±23.90 µg/mL, respectively. The concentrations of salivary UA in GPs were significantly higher than those in HVs ( p < 0.001). This method was also expected to monitor the hyperuricemia and other metabolic disorders in the future.
  • Títulos relacionados: https://www.revistas.usp.br/bjps/article/view/208737/191795
  • Editor: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
  • Data de criação/publicação: 2023-02-28
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.