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Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes

Gibney, Patrick A ; Lu, Charles ; Caudy, Amy A ; Hess, David C ; Botstein, David

Proceedings of the National Academy of Sciences - PNAS, 2013-11, Vol.110 (46), p.E4393-E4402 [Periódico revisado por pares]

United States: National Academy of Sciences

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  • Título:
    Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes
  • Autor: Gibney, Patrick A ; Lu, Charles ; Caudy, Amy A ; Hess, David C ; Botstein, David
  • Assuntos: Biological Sciences ; Chromatin ; DNA Barcoding, Taxonomic ; DNA Primers - genetics ; Gene Deletion ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Fungal - genetics ; Genes ; Genetic Markers - genetics ; Genetics ; Heat ; Heat-Shock Response - genetics ; High-Throughput Nucleotide Sequencing ; Metabolic Networks and Pathways - genetics ; Molecular Sequence Annotation ; PNAS Plus ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Signal Transduction - genetics ; Stress ; Systems Biology - methods ; Yeast
  • É parte de: Proceedings of the National Academy of Sciences - PNAS, 2013-11, Vol.110 (46), p.E4393-E4402
  • Notas: http://dx.doi.org/10.1073/pnas.1318100110
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Contributed by David Botstein, September 30, 2013 (sent for review August 25, 2013)
    2Present address: Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3E1.
    3A.A.C. and D.C.H. contributed equally to this work.
    Author contributions: P.A.G., C.L., A.A.C., D.C.H., and D.B. designed research; P.A.G., C.L., A.A.C., and D.C.H. performed research; P.A.G., C.L., A.A.C., and D.C.H. contributed new reagents/analytical tools; P.A.G., C.L., A.A.C., D.C.H., and D.B. analyzed data; and P.A.G., C.L., A.A.C., D.C.H., and D.B. wrote the paper.
    1P.A.G. and C.L. contributed equally to this work.
  • Descrição: Genome-wide gene-expression studies have shown that hundreds of yeast genes are induced or repressed transiently by changes in temperature; many are annotated to stress response on this basis. To obtain a genome-scale assessment of which genes are functionally important for innate and/or acquired thermotolerance, we combined the use of a barcoded pool of ∼4,800 nonessential, prototrophic Saccharomyces cerevisiae deletion strains with Illumina-based deep-sequencing technology. As reported in other recent studies that have used deletion mutants to study stress responses, we observed that gene deletions resulting in the highest thermosensitivity generally are not the same as those transcriptionally induced in response to heat stress. Functional analysis of identified genes revealed that metabolism, cellular signaling, and chromatin regulation play roles in regulating thermotolerance and in acquired thermotolerance. However, for most of the genes identified, the molecular mechanism behind this action remains unclear. In fact, a large fraction of identified genes are annotated as having unknown functions, further underscoring our incomplete understanding of the response to heat shock. We suggest that survival after heat shock depends on a small number of genes that function in assessing the metabolic health of the cell and/or regulate its growth in a changing environment.
  • Editor: United States: National Academy of Sciences
  • Idioma: Inglês
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