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PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection

Abu Dail, Yaser ; Daas, Loay ; Flockerzi, Elias ; Munteanu, Cristian ; Kahlert, Julian ; Smola, Sigrun ; Seitz, Berthold

Journal of medical virology, 2024-03, Vol.96 (3), p.e29538-n/a [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection
Autor: Abu Dail, Yaser ; Daas, Loay ; Flockerzi, Elias ; Munteanu, Cristian ; Kahlert, Julian ; Smola, Sigrun ; Seitz, Berthold
Assuntos: aqueous humor ; Aqueous Humor - chemistry ; Aqueous humour ; Cornea ; corneal endothelial allograft rejection ; Corneal transplantation ; Cross-Sectional Studies ; Cytomegalovirus ; Cytomegalovirus - genetics ; Cytomegalovirus Infections ; DNA ; DNA viruses ; DNA, Viral - analysis ; DNA, Viral - genetics ; Epstein-Barr virus ; Epstein-Barr Virus Infections ; Eye surgery ; Eyes ; Graft rejection ; Graft Rejection - diagnosis ; Herpes simplex ; Herpes viruses ; Herpesviridae Infections - diagnosis ; Herpesvirus 3, Human - genetics ; Herpesvirus 4, Human - genetics ; herpesviruses ; Humans ; Keratitis ; Keratitis, Herpetic ; keratoplasty ; Nucleotide sequence ; Patients ; PCR testing ; Polymerase Chain Reaction ; Rejection ; Retrospective Studies ; Simplexvirus - genetics ; Statistical analysis ; Varicella ; Viral replication ; Viruses
É parte de: Journal of medical virology, 2024-03, Vol.96 (3), p.e29538-n/a
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Descrição: To compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross‐sectional study with two‐group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella‐zoster virus (VZV), cytomegalovirus (CMV), and Epstein‐Barr virus (EBV). PCR test results were compared between the two groups (with/without AGR). Another sub‐analysis examined the results of patients without a history of herpetic keratitis. A total of 8% of eyes with clinical AGR (9/108) had a positive PCR result for one of the herpesviruses (HSV:3, CMV:3, EBV:2, VZV:1). All patients in the group without AGR had negative PCR results for all previous viruses (0/136). The difference was statistically significant (p < 0.001). The sub‐analysis of eyes without a history of herpetic keratitis also revealed significantly more positive herpes PCR results (7/87) in eyes with AGR than in eyes without AGR (0/42, p = 0.005). Clinical AGR after keratoplasty shows a significant correlation to viral replication. Herpetic infection and AGR could occur simultaneously and act synergistically. Timely differentiation between active herpetic infection and/or AGR is pivotal for proper treatment and graft preservation.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection

Abu Dail, Yaser ; Daas, Loay ; Flockerzi, Elias ; Munteanu, Cristian ; Kahlert, Julian ; Smola, Sigrun ; Seitz, Berthold

United States: Wiley Subscription Services, Inc

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