skip to main content
Idioma:

Sensitive β-substituted aliphatic Ile/Val-Xxx and aromatic Phe/Tyr/His-Xxx residues to form [a]+ ions in high energy CID of peptides

Sekiya, Sadanori ; Yamakoshi, Mariko ; Iwamoto, Shinichi ; Tanaka, Koichi ; Takayama, Mitsuo

International journal of mass spectrometry, 2019-11, Vol.445, p.116195, Article 116195 [Periódico revisado por pares]

Elsevier B.V

Texto completo disponível

Citações Citado por
  • Título:
    Sensitive β-substituted aliphatic Ile/Val-Xxx and aromatic Phe/Tyr/His-Xxx residues to form [a]+ ions in high energy CID of peptides
  • Autor: Sekiya, Sadanori ; Yamakoshi, Mariko ; Iwamoto, Shinichi ; Tanaka, Koichi ; Takayama, Mitsuo
  • Assuntos: A-ion ; High-energy CID ; Hydrogen loss ; Peptides ; Val and Ile residues
  • É parte de: International journal of mass spectrometry, 2019-11, Vol.445, p.116195, Article 116195
  • Descrição: Residue-specific cleavages resulting in the formation of [a]+ ions of peptides have been examined using 20 keV high-energy (high-E) collision-induced dissociation (CID) with a tandem time-of-flight (TOF/TOF) instrument. High-E CID resulted in relatively sensitive cleavage at the Cα-C bond of β-substituted aliphatic Val/Ile-Xxx and aromatic Phe/Tyr/His-Xxx residues when an Arg residue was located on the N-terminal side. The sensitive aromatic Phe/Tyr/His residues and insensitive Gly residue in high-E CID are similar to other methods, such as ultraviolet photodissociation (UVPD) of [M+H]+, low-E CID of [M].+ and MALDI-ISD with a hydrogen-abstracting matrix. The properties of the Val/Ile residues are not necessarily common to the same methods. The high-sensitivity of Val/Ile and Phe/Tyr/His residues to high-E CID may be due to high-energy single collision between target gas and the bulky sidechains of these residues. It is suggested that from high-E CID and MALDI-ISD experiments the formation of [a]+ ions is essentially connected with the loss of atomic hydrogen from protonated peptides [M+H]+, and that high-E CID results in the loss of hydrogen from the backbone amide nitrogen rather than the site of the β-carbon of sidechains. [Display omitted] •Val and Ile residues can form intense [a]+ ions in high-energy (E) CID of peptides. .•The sensitivity of residues to high-E CID is common to other techniques. .•Loss of atomic hydrogen from [M+H]+ is crucial for formation of [a]+ ions. .•The loss of hydrogen mainly takes place from backbone amide nitrogen. .
  • Editor: Elsevier B.V
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.