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Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies

Danhier, P. ; De Preter, G. ; Boutry, S. ; Mahieu, I. ; Leveque, P. ; Magat, J. ; Haufroid, V. ; Sonveaux, P. ; Bouzin, C. ; Feron, O. ; Muller, R. N. ; Jordan, B. F. ; Gallez, B.

Contrast media and molecular imaging, 2012-05, Vol.7 (3), p.302-307 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

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Título:
Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies
Autor: Danhier, P. ; De Preter, G. ; Boutry, S. ; Mahieu, I. ; Leveque, P. ; Magat, J. ; Haufroid, V. ; Sonveaux, P. ; Bouzin, C. ; Feron, O. ; Muller, R. N. ; Jordan, B. F. ; Gallez, B.
Assuntos: Adenocarcinoma - chemistry ; Adenocarcinoma - pathology ; Animals ; cell labeling ; Cells, Cultured ; Electron Spin Resonance Spectroscopy ; EPR ; Ferric Compounds - analysis ; Ferric Compounds - metabolism ; Fibroblasts - chemistry ; Fibroblasts - cytology ; iron oxide particles ; Kidney Neoplasms - chemistry ; Kidney Neoplasms - pathology ; Kinetics ; Limit of Detection ; Luciferases - metabolism ; Magnetic Resonance Imaging ; Mass Spectrometry ; Melanoma, Experimental - chemistry ; Melanoma, Experimental - pathology ; Mice ; Microscopy, Fluorescence
É parte de: Contrast media and molecular imaging, 2012-05, Vol.7 (3), p.302-307
Notas: Fonds National de la Recherche Scientifique
Saint-Luc Foundation
ark:/67375/WNG-663MHTZQ-F
Pôle d'attraction Interuniversitaire PAI VI - No. P6/38; No. P6/29
ArticleID:CMMI497
istex:5F0B7A6FAF0187A6A94C74E726A565D16D0E8AB7
Actions de Recherches Concertées-Communauté Française de Belgique - No. ARC 09/14-020
Fonds Joseph Maisin
Fondation contre le Cancer
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: ABSTRACT MRI cell tracking is a promising technique to track various cell types (stem cells, tumor cells, etc.) in living animals. Usually, cells are incubated with iron oxides (T2 contrast agent) in order to take up the particles before being injected in vivo. Iron oxide quantification is important in such studies for validating the labeling protocols and assessing the dilution of the particles with cell proliferation. We here propose to implement electron paramagnetic resonance (EPR) as a very sensitive method to quantify iron oxide concentration in cells. Iron oxide particles exhibit a unique EPR spectrum, which directly reflects the number of particles in a sample. In order to compare EPR with existing methods (Perls's Prussian blue reaction, ICP‐MS and fluorimetry), we labeled tumor cells (melanoma and renal adenocarcinoma cell lines) and fibroblasts with fluorescent iron oxide particles, and determined the limits of detection of the different techniques. We show that EPR is a very sensitive technique and is specific for iron oxide quantification as measurements are not affected by endogenous iron. As a consequence, EPR is well adapted to perform ex vivo analysis of tissues after cell tracking experiments in order to confirm MRI results. Copyright © 2012 John Wiley & Sons, Ltd. Electron paramagnetic resonance is a specific and sensitive tool for assessing the intracellular iron oxide content after labeling procedures. The technique is reliable compared with other existing methods and allows ex vivo iron oxide quantification after MRI cell tracking experiments.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

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Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies

Danhier, P. ; De Preter, G. ; Boutry, S. ; Mahieu, I. ; Leveque, P. ; Magat, J. ; Haufroid, V. ; Sonveaux, P. ; Bouzin, C. ; Feron, O. ; Muller, R. N. ; Jordan, B. F. ; Gallez, B.

Chichester, UK: John Wiley & Sons, Ltd

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