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Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential

Takov, Kaloyan ; He, Zhenhe ; Johnston, Harvey E. ; Timms, John F. ; Guillot, Pascale V. ; Yellon, Derek M. ; Davidson, Sean M.

Basic research in cardiology, 2020-05, Vol.115 (3), p.26-26, Article 26 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential
Autor: Takov, Kaloyan ; He, Zhenhe ; Johnston, Harvey E. ; Timms, John F. ; Guillot, Pascale V. ; Yellon, Derek M. ; Davidson, Sean M.
Assuntos: 1-Phosphatidylinositol 3-kinase ; Amniotic fluid ; Amniotic Fluid - cytology ; Animals ; Cardiology ; Cardiomyocytes ; Cardiotonic Agents - metabolism ; Cell adhesion & migration ; Cell migration ; Cell Movement ; Chemotaxis ; Computer simulation ; Endothelial cells ; Extracellular vesicles ; Extracellular Vesicles - metabolism ; Heart attacks ; Humans ; Impurities ; Ischemia ; Kinases ; Medicine ; Medicine & Public Health ; Mesenchymal stem cells ; Mesenchymal Stem Cells - metabolism ; Mesenchyme ; Myocardial infarction ; Myocytes, Cardiac - metabolism ; Myocytes, Cardiac - pathology ; Original Contribution ; Phosphatidylinositol 3-Kinase - metabolism ; Rats ; Reperfusion ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Size exclusion chromatography ; Size reduction ; Stem cell transplantation ; Stem cells ; Stromal cells ; Vesicles
É parte de: Basic research in cardiology, 2020-05, Vol.115 (3), p.26-26, Article 26
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Mesenchymal stromal cells (MSCs) exhibit antiapoptotic and proangiogenic functions in models of myocardial infarction which may be mediated by secreted small extracellular vesicles (sEVs). However, MSCs have frequently been harvested from aged or diseased patients, while the isolated sEVs often contain high levels of impurities. Here, we studied the cardioprotective and proangiogenic activities of size-exclusion chromatography-purified sEVs secreted from human foetal amniotic fluid stem cells (SS-hAFSCs), possessing superior functional potential to that of adult MSCs. We demonstrated for the first time that highly pure (up to 1.7 × 10 10 particles/µg protein) and thoroughly characterised SS-hAFSC sEVs protect rat hearts from ischaemia–reperfusion injury in vivo when administered intravenously prior to reperfusion (38 ± 9% infarct size reduction, p < 0.05). SS-hAFSC sEVs did not protect isolated primary cardiomyocytes in models of simulated ischaemia–reperfusion injury in vitro, indicative of indirect cardioprotective effects. SS-hAFSC sEVs were not proangiogenic in vitro, although they markedly stimulated endothelial cell migration. Additionally, sEVs were entirely responsible for the promigratory effects of the medium conditioned by SS-hAFSC. Mechanistically, sEV-induced chemotaxis involved phosphatidylinositol 3-kinase (PI3K) signalling, as its pharmacological inhibition in treated endothelial cells reduced migration by 54 ± 7% ( p < 0.001). Together, these data indicate that SS-hAFSC sEVs have multifactorial beneficial effects in a myocardial infarction setting.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential

Takov, Kaloyan ; He, Zhenhe ; Johnston, Harvey E. ; Timms, John F. ; Guillot, Pascale V. ; Yellon, Derek M. ; Davidson, Sean M.

Berlin/Heidelberg: Springer Berlin Heidelberg

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