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Physiology of Consumption of Human Milk Oligosaccharides by Infant Gut-associated Bifidobacteria

Asakuma, Sadaki ; Hatakeyama, Emi ; Urashima, Tadasu ; Yoshida, Erina ; Katayama, Takane ; Yamamoto, Kenji ; Kumagai, Hidehiko ; Ashida, Hisashi ; Hirose, Junko ; Kitaoka, Motomitsu

The Journal of biological chemistry, 2011-10, Vol.286 (40), p.34583-34592 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Physiology of Consumption of Human Milk Oligosaccharides by Infant Gut-associated Bifidobacteria
Autor: Asakuma, Sadaki ; Hatakeyama, Emi ; Urashima, Tadasu ; Yoshida, Erina ; Katayama, Takane ; Yamamoto, Kenji ; Kumagai, Hidehiko ; Ashida, Hisashi ; Hirose, Junko ; Kitaoka, Motomitsu
Assuntos: Adult ; Bacteria ; Bifidobacteria ; Bifidobacteriales Infections - microbiology ; Bifidobacterium - metabolism ; Carbohydrate Metabolism ; Carbohydrates - chemistry ; Chromatography, High Pressure Liquid - methods ; Enzymes ; Female ; Gastrointestinal Tract - microbiology ; Gut Ecosystem ; HPLC ; Human Milk Oligosaccharides ; Humans ; Infant, Newborn ; Intestines - microbiology ; Japan ; Microbiology ; Milk, Human - metabolism ; Molecular Sequence Data ; Monosaccharides ; Oligosaccharide ; Oligosaccharides - chemistry ; Oligosaccharides - metabolism ; Time Factors
É parte de: The Journal of biological chemistry, 2011-10, Vol.286 (40), p.34583-34592
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The bifidogenic effect of human milk oligosaccharides (HMOs) has long been known, yet the precise mechanism underlying it remains unresolved. Recent studies show that some species/subspecies of Bifidobacterium are equipped with genetic and enzymatic sets dedicated to the utilization of HMOs, and consequently they can grow on HMOs; however, the ability to metabolize HMOs has not been directly linked to the actual metabolic behavior of the bacteria. In this report, we clarify the fate of each HMO during cultivation of infant gut-associated bifidobacteria. Bifidobacterium bifidum JCM1254, Bifidobacterium longum subsp. infantis JCM1222, Bifidobacterium longum subsp. longum JCM1217, and Bifidobacterium breve JCM1192 were selected for this purpose and were grown on HMO media containing a main neutral oligosaccharide fraction. The mono- and oligosaccharides in the spent media were labeled with 2-anthranilic acid, and their concentrations were determined at various incubation times using normal phase high performance liquid chromatography. The results reflect the metabolic abilities of the respective bifidobacteria. B. bifidum used secretory glycosidases to degrade HMOs, whereas B. longum subsp. infantis assimilated all HMOs by incorporating them in their intact forms. B. longum subsp. longum and B. breve consumed lacto-N-tetraose only. Interestingly, B. bifidum left degraded HMO metabolites outside of the cell even when the cells initiate vegetative growth, which indicates that the different species/subspecies can share the produced sugars. The predominance of type 1 chains in HMOs and the preferential use of type 1 HMO by infant gut-associated bifidobacteria suggest the coevolution of the bacteria with humans.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Physiology of Consumption of Human Milk Oligosaccharides by Infant Gut-associated Bifidobacteria

Asakuma, Sadaki ; Hatakeyama, Emi ; Urashima, Tadasu ; Yoshida, Erina ; Katayama, Takane ; Yamamoto, Kenji ; Kumagai, Hidehiko ; Ashida, Hisashi ; Hirose, Junko ; Kitaoka, Motomitsu

United States: Elsevier Inc

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