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Spectroscopic studies of the cytochrome P450 reaction mechanisms

Mak, Piotr J. ; Denisov, Ilia G.

Biochimica et biophysica acta, 2018-01, Vol.1866 (1), p.178-204 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Spectroscopic studies of the cytochrome P450 reaction mechanisms
Autor: Mak, Piotr J. ; Denisov, Ilia G.
Assuntos: Biocatalysis ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P450 ; Electron Spin Resonance Spectroscopy - instrumentation ; Electron Spin Resonance Spectroscopy - methods ; EPR spectroscopy ; Free Radicals - chemistry ; Freezing ; Glycerol - chemistry ; Heme - chemistry ; Iron - chemistry ; Magnetic Resonance Spectroscopy - instrumentation ; Magnetic Resonance Spectroscopy - methods ; Models, Molecular ; Nanodiscs ; NMR spectroscopy ; Oxidation-Reduction ; Oxygen - chemistry ; Protein Structure, Secondary ; Resonance Raman spectroscopy ; Spectrum Analysis, Raman - instrumentation ; Spectrum Analysis, Raman - methods ; UV–Vis spectroscopy
É parte de: Biochimica et biophysica acta, 2018-01, Vol.1866 (1), p.178-204
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-2
Descrição: The cytochrome P450 monooxygenases (P450s) are thiolate heme proteins that can, often under physiological conditions, catalyze many distinct oxidative transformations on a wide variety of molecules, including relatively simple alkanes or fatty acids, as well as more complex compounds such as steroids and exogenous pollutants. They perform such impressive chemistry utilizing a sophisticated catalytic cycle that involves a series of consecutive chemical transformations of heme prosthetic group. Each of these steps provides a unique spectral signature that reflects changes in oxidation or spin states, deformation of the porphyrin ring or alteration of dioxygen moieties. For a long time, the focus of cytochrome P450 research was to understand the underlying reaction mechanism of each enzymatic step, with the biggest challenge being identification and characterization of the powerful oxidizing intermediates. Spectroscopic methods, such as electronic absorption (UV–Vis), electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), electron nuclear double resonance (ENDOR), Mössbauer, X-ray absorption (XAS), and resonance Raman (rR), have been useful tools in providing multifaceted and detailed mechanistic insights into the biophysics and biochemistry of these fascinating enzymes. The combination of spectroscopic techniques with novel approaches, such as cryoreduction and Nanodisc technology, allowed for generation, trapping and characterizing long sought transient intermediates, a task that has been difficult to achieve using other methods. Results obtained from the UV–Vis, rR and EPR spectroscopies are the main focus of this review, while the remaining spectroscopic techniques are briefly summarized. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone. [Display omitted]
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Spectroscopic studies of the cytochrome P450 reaction mechanisms

Mak, Piotr J. ; Denisov, Ilia G.

Netherlands: Elsevier B.V

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