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Analysis of Serum miRNA in Glioblastoma Patients: CD44-Based Enrichment of Extracellular Vesicles Enhances Specificity for the Prognostic Signature

Tzaridis, Theophilos ; Reiners, Katrin S ; Weller, Johannes ; Bachurski, Daniel ; Schäfer, Niklas ; Schaub, Christina ; Hallek, Michael ; Scheffler, Björn ; Glas, Martin ; Herrlinger, Ulrich ; Wild, Stefan ; Coch, Christoph ; Hartmann, Gunther

International journal of molecular sciences, 2020-10, Vol.21 (19), p.7211 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Analysis of Serum miRNA in Glioblastoma Patients: CD44-Based Enrichment of Extracellular Vesicles Enhances Specificity for the Prognostic Signature
Autor: Tzaridis, Theophilos ; Reiners, Katrin S ; Weller, Johannes ; Bachurski, Daniel ; Schäfer, Niklas ; Schaub, Christina ; Hallek, Michael ; Scheffler, Björn ; Glas, Martin ; Herrlinger, Ulrich ; Wild, Stefan ; Coch, Christoph ; Hartmann, Gunther
Assuntos: Adult ; Aged ; Biomarkers ; Biomarkers, Tumor - blood ; Brain cancer ; CD44 ; CD44 antigen ; Chromatography ; Disease-Free Survival ; extracellular vesicles ; Extracellular Vesicles - genetics ; Female ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic - genetics ; Genetic aspects ; Glioblastoma ; Glioblastoma - blood ; Glioblastoma - genetics ; Glioblastoma - pathology ; Glioblastoma multiforme ; Health aspects ; Humans ; Hyaluronan Receptors - blood ; Immunoprecipitation ; Male ; Medical prognosis ; Methods ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; miRNA ; Patients ; Plasma ; Prognosis ; Proteins ; RNA sequencing ; Size exclusion chromatography ; Tumors ; Vesicles ; Young Adult
É parte de: International journal of molecular sciences, 2020-10, Vol.21 (19), p.7211
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These authors contributed equally to this work.
Descrição: Glioblastoma is a devastating disease, for which biomarkers allowing a prediction of prognosis are urgently needed. microRNAs have been described as potentially valuable biomarkers in cancer. Here, we studied a panel of microRNAs in extracellular vesicles (EVs) from the serum of glioblastoma patients and evaluated their correlation with the prognosis of these patients. The levels of 15 microRNAs in EVs that were separated by size-exclusion chromatography were studied by quantitative real-time PCR, followed by CD44 immunoprecipitation (SEC + CD44), and compared with those from the total serum of glioblastoma patients ( = 55) and healthy volunteers ( = 10). Compared to total serum, we found evidence for the enrichment of miR-21-3p and miR-106a-5p and, conversely, lower levels of miR-15b-3p, in SEC + CD44 EVs. miR-15b-3p and miR-21-3p were upregulated in glioblastoma patients compared to healthy subjects. A significant correlation with survival of the patients was found for levels of miR-15b-3p in total serum and miR-15b-3p, miR-21-3p, miR-106a-5p, and miR-328-3p in SEC + CD44 EVs. Combining miR-15b-3p in serum or miR-106a-5p in SEC + CD44 EVs with any one of the other three microRNAs in SEC + CD44 EVs allowed for a prognostic stratification of glioblastoma patients. We have thus identified four microRNAs in glioblastoma patients whose levels, in combination, can predict the prognosis for these patients.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Analysis of Serum miRNA in Glioblastoma Patients: CD44-Based Enrichment of Extracellular Vesicles Enhances Specificity for the Prognostic Signature

Tzaridis, Theophilos ; Reiners, Katrin S ; Weller, Johannes ; Bachurski, Daniel ; Schäfer, Niklas ; Schaub, Christina ; Hallek, Michael ; Scheffler, Björn ; Glas, Martin ; Herrlinger, Ulrich ; Wild, Stefan ; Coch, Christoph ; Hartmann, Gunther

Switzerland: MDPI AG

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