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Cellulase Production by Aspergillus japonicus URM5620 Using Waste from Castor Bean ( Ricinus communis L.) Under Solid-State Fermentation

Herculano, Polyanna ; Porto, Tatiana ; Moreira, Keila ; Pinto, Gustavo ; Souza-Motta, Cristina ; Porto, Ana

Applied Biochemistry and Biotechnology, 2011, Vol.165(3), pp.1057-1067 [Periódico revisado por pares]

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  • Título:
    Cellulase Production by Aspergillus japonicus URM5620 Using Waste from Castor Bean ( Ricinus communis L.) Under Solid-State Fermentation
  • Autor: Herculano, Polyanna ; Porto, Tatiana ; Moreira, Keila ; Pinto, Gustavo ; Souza-Motta, Cristina ; Porto, Ana
  • Assuntos: Aspergillus japonicus ; Cellulolytic activities ; Castor bean meal ; Solid-state fermentation ; Optimum activity ; Stability
  • É parte de: Applied Biochemistry and Biotechnology, 2011, Vol.165(3), pp.1057-1067
  • Descrição: The activity of β-glucosidase (βG), total cellulase (FPase) and endoglucanase (CMCase), produced by Aspergillus japonicus URM5620, was studied on solid-state fermentation using castor bean meal as substrate. The effect of the substrate amount, initial moisture, pH, and temperature on cellulase production was studied using a full factorial design (2 4 ). The maximum βG, FPase, and CMCase activity was 88.3, 953.4, and 191.6 U/g dry substrate, respectively. The best enzyme activities for all three enzymes were obtained at the same conditions with 5.0 g of substrate, initial moisture 15% at 25 °C and pH 6.0 with 120 h of fermentation. The optimum activity for FPase and CMCase was found at pH 3.0 at an optimum temperature of 50 °C for FPase and of 55 °C for CMCase. The cellulases were stable in the range of pH 3.0–10.0 at 50 °C temperature. The enzyme production optimization demonstrated clearly the impact of the process parameters on the yield of the cellulolytic enzymes.
  • Idioma: Inglês

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