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Cloning and Characterization of the RubisCO Activase Gene from Pinus massoniana

Pan, Ting ; Zhang, Fengkai ; Sheng, Lu ; Wang, Xiaofeng ; Ruan, Weicheng ; Zhu, Peihuang ; Guo, Tianwei ; Ji, Kongshu

Plant molecular biology reporter, 2023-03, Vol.41 (1), p.81-91 [Periódico revisado por pares]

New York: Springer US

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Título:
Cloning and Characterization of the RubisCO Activase Gene from Pinus massoniana
Autor: Pan, Ting ; Zhang, Fengkai ; Sheng, Lu ; Wang, Xiaofeng ; Ruan, Weicheng ; Zhu, Peihuang ; Guo, Tianwei ; Ji, Kongshu
Assuntos: Bioinformatics ; Biomedical and Life Sciences ; Cloning ; Expression vectors ; Genes ; Homology ; Life Sciences ; Metabolomics ; Open reading frames ; Original Article ; Photoinhibition ; Photosynthesis ; Pine trees ; Pinus massoniana ; Plant Breeding/Biotechnology ; Plant Sciences ; Plant species ; Proteomics ; Respiration ; Ribulose-bisphosphate carboxylase ; Tobacco ; Transgenic plants
É parte de: Plant molecular biology reporter, 2023-03, Vol.41 (1), p.81-91
Descrição: Pinus massoniana ( P. massoniana ) is an important fast-growing tree species in China. To explore photosynthesis-related gene resources and find possible targets for high photosynthetic-efficiency breeding of P. massoniana , we cloned two full-length cDNAs with conserved sequences of RubisCO activase (RCA) genes from P. massoniana . The RCA enzyme is important in maintaining the activity of RubisCO in vivo. Two genes, named PmRCA1 and PmRCA2 , showed high similarity to RCA genes from other species and were identical to each other except for a 137 bp intervening sequence in the open reading frame (ORF) of PmRCA1 , which led to different protein products, herein referred to as Pm RCA1 and Pm RCA2, respectively. Pm RCA1 and Pm RCA2 showed high homology to RCA of Pinus halepensis , and Pm RCA1 had two cysteine residues in its carboxyl-terminal extension that were unique to the large RCA isoform. To characterize the function of PmRCA1 and PmRCA2 , plant expression vectors driven by the constitutive 35S promoter were transformed in tobacco plants. Transgenic plants of PmRCA2 were grown better than wild tobacco plants and had significantly more leaves. Overexpression of PmRCA2 increased the maximum net photosynthetic rate and dark respiration rate of tobacco by 16.49% and 81.85%, respectively. Overexpression of PmRCA1 increased the maximum net photosynthetic rate and dark respiration rate of tobacco more than overexpression of PmRCA2 , but it simultaneously enhanced photoinhibition of photosynthesis.
Editor: New York: Springer US
Idioma: Inglês

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Cloning and Characterization of the RubisCO Activase Gene from Pinus massoniana

Pan, Ting ; Zhang, Fengkai ; Sheng, Lu ; Wang, Xiaofeng ; Ruan, Weicheng ; Zhu, Peihuang ; Guo, Tianwei ; Ji, Kongshu

New York: Springer US

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