Idioma:

Genistein inhibits Ca2+ influx and glutamate release from hippocampal synaptosomes: putative non-specific effects

PEREIRA, Daniela B ; CARVALHO, Arsélio P ; DUARTE, Carlos B

Neurochemistry international, 2003, Vol.42 (2), p.179-188 [Periódico revisado por pares]

Oxford: Elsevier

Texto completo disponível

Citações Citado por

Enviar para

Título:
Genistein inhibits Ca2+ influx and glutamate release from hippocampal synaptosomes: putative non-specific effects
Autor: PEREIRA, Daniela B ; CARVALHO, Arsélio P ; DUARTE, Carlos B
Assuntos: Animals ; Biological and medical sciences ; Calcium - metabolism ; Calcium Channel Blockers - pharmacology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Genistein - pharmacology ; Glutamic Acid - metabolism ; Hippocampus - drug effects ; Hippocampus - metabolism ; Immunoblotting ; In Vitro Techniques ; Male ; Nerve Endings - drug effects ; Nerve Endings - enzymology ; omega-Agatoxin IVA - pharmacology ; Phosphorylation - drug effects ; Potassium Chloride - pharmacology ; Protein Tyrosine Phosphatases - antagonists & inhibitors ; Protein-Tyrosine Kinases - antagonists & inhibitors ; Rats ; Rats, Wistar ; Synaptosomes - drug effects ; Synaptosomes - metabolism
É parte de: Neurochemistry international, 2003, Vol.42 (2), p.179-188
Descrição: The role of protein tyrosine kinases on glutamate release was investigated by determining the effect of broad range inhibitors of tyrosine kinases on the release of glutamate from rat hippocampal synaptosomes. We found that lavendustin A and herbimycin A did not inhibit glutamate release stimulated by 15 mM KCl, but genistein, also a broad range inhibitor of tyrosine kinases did inhibit the intracellular Ca(2+) concentration response to KCl and, concomitantly, decreased glutamate release evoked by the same stimulus, in a dose-dependent manner. These effects were not observed with the inactive analogue genistin. Therefore, we investigated the mechanism whereby genistein modulates Ca(2+) influx and glutamate release. Studies with voltage-gated Ca(2+) channel inhibitors showed that omega-conotoxin GVIA did not further inhibit glutamate release or the Ca(2+) influx stimulated by KCl in the presence of genistein. This tyrosine kinase inhibitor and omega-agatoxin IVA had a partially additive effect on those events. Nitrendipine did not reduce significantly the KCl-induced responses. Genistein further reduced Ca(2+) influx in response to KCl in the presence of nitrendipine, omega-conotoxin GVIA and omega-agatoxin IVA, simultaneously. The effect of tyrosine phosphatase inhibitors was also tested on the influx of Ca(2+) and on glutamate release stimulated by KCl-depolarization. We found that the broad range inhibitors sodium orthovanadate and dephostatin did not significantly affect these KCl-evoked events. Our results suggest that genistein inhibits glutamate release and Ca(2+) influx in response to KCl independently of tyrosine kinase inhibition, and that tyrosine kinases and phosphatases are not key regulators of glutamate release in hippocampal nerve terminals.
Editor: Oxford: Elsevier
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Genistein inhibits Ca2+ influx and glutamate release from hippocampal synaptosomes: putative non-specific effects

PEREIRA, Daniela B ; CARVALHO, Arsélio P ; DUARTE, Carlos B

Oxford: Elsevier

Buscando em bases de dados remotas. Favor aguardar.