Idioma:

11β-HSD1 Inhibitor Alleviates Non-Alcoholic Fatty Liver Disease by Activating the AMPK/SIRT1 Signaling Pathway

Chen, Ying ; Li, Jiali ; Zhang, Meng ; Yang, Wei ; Qin, Wenqi ; Zheng, Qinzhou ; Chu, Yanhui ; Wu, Yan ; Wu, Dan ; Yuan, Xiaohuan

Nutrients, 2022-06, Vol.14 (11), p.2358 [Periódico revisado por pares]

Switzerland: MDPI AG

Texto completo disponível

Citações Citado por

Enviar para

Título:
11β-HSD1 Inhibitor Alleviates Non-Alcoholic Fatty Liver Disease by Activating the AMPK/SIRT1 Signaling Pathway
Autor: Chen, Ying ; Li, Jiali ; Zhang, Meng ; Yang, Wei ; Qin, Wenqi ; Zheng, Qinzhou ; Chu, Yanhui ; Wu, Yan ; Wu, Dan ; Yuan, Xiaohuan
Assuntos: 11-beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ; AMP-activated protein kinase ; anti-inflammatory ; Cholesterol ; Curcumin ; Enzyme inhibitors ; Enzymes ; Fatty liver ; Fibrosis ; High fat diet ; Homeostasis ; Inflammation ; Insulin ; Insulin resistance ; Kinases ; Laboratory animals ; lipid metabolism ; Lipids ; Liver ; Liver diseases ; Metabolism ; non-alcoholic fatty liver disease (NAFLD) ; Proteins ; Signal transduction ; Signaling ; SIRT1 protein ; Steatosis ; Streptozocin
É parte de: Nutrients, 2022-06, Vol.14 (11), p.2358
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: We investigated the effect of an 11β-HSD1 inhibitor (H8) on hepatic steatosis and its mechanism of action. Although H8, a curcumin derivative, has been shown to alleviate insulin resistance, its effect on non-alcoholic fatty liver disease (NAFLD) remains unknown. Rats were fed a high-fat diet (HFD) for 8 weeks, intraperitoneally injected with streptozotocin (STZ) to induce NAFLD, and, then, treated with H8 (3 or 6 mg/kg/day) or curcumin (6 mg/kg/day) for 4 weeks, to evaluate the effects of H8 on NAFLD. H8 significantly alleviated HFD+STZ-induced lipid accumulation, fibrosis, and inflammation as well as improved liver function. Moreover, 11β-HSD1 overexpression was established by transfecting animals and HepG2 cells with lentivirus, carrying the 11β-HSD1 gene, to confirm that H8 improved NAFLD, by reducing 11β-HSD1. An AMP-activated protein kinase (AMPK) inhibitor (Compound C, 10 μM for 2 h) was used to confirm that H8 increased AMPK, by inhibiting 11β-HSD1, thereby restoring lipid metabolic homeostasis. A silencing-related enzyme 1 (SIRT1) inhibitor (EX572, 10 μM for 4 h) and a SIRT1 activator (SRT1720, 1 μM for 4 h) were used to confirm that H8 exerted anti-inflammatory effects, by elevating SIRT1 expression. Our findings demonstrate that H8 alleviates hepatic steatosis, by inhibiting 11β-HSD1, which activates the AMPK/SIRT1 signaling pathway.
Editor: Switzerland: MDPI AG
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

11β-HSD1 Inhibitor Alleviates Non-Alcoholic Fatty Liver Disease by Activating the AMPK/SIRT1 Signaling Pathway

Chen, Ying ; Li, Jiali ; Zhang, Meng ; Yang, Wei ; Qin, Wenqi ; Zheng, Qinzhou ; Chu, Yanhui ; Wu, Yan ; Wu, Dan ; Yuan, Xiaohuan

Switzerland: MDPI AG

Buscando em bases de dados remotas. Favor aguardar.