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Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtusa Sieb. et Zucc

Taniguchi, T ; Kurita, M ; Ohmiya, Y ; Kondo, T

Plant cell reports, 2005-03, Vol.23 (12), p.796-802 [Periódico revisado por pares]

Germany: Springer Nature B.V

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Título:
Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtusa Sieb. et Zucc
Autor: Taniguchi, T ; Kurita, M ; Ohmiya, Y ; Kondo, T
Assuntos: Agrobacterium tumefaciens ; Agrobacterium tumefaciens - genetics ; Bacteria ; Biomarkers ; Cells, Cultured ; Chamaecyparis - embryology ; Chamaecyparis - genetics ; Chamaecyparis obtusa ; Coculture Techniques ; conifers ; Gene Expression Regulation, Plant - genetics ; Gene Transfer Techniques ; Genes, Reporter ; genetic transformation ; genetic vectors ; Genetic Vectors - genetics ; Genome, Plant ; Green Fluorescent Proteins - genetics ; Kanamycin Kinase - genetics ; Microbiology ; micropropagation ; molecular sequence data ; nucleotide sequences ; plant regeneration ; Plants, Genetically Modified - embryology ; Plants, Genetically Modified - genetics ; Regeneration - genetics ; Seeds - embryology ; Seeds - genetics ; somatic embryogenesis ; somatic embryos ; Transformation, Genetic - genetics ; Transgenes - genetics ; Transgenic plants
É parte de: Plant cell reports, 2005-03, Vol.23 (12), p.796-802
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A genetic transformation procedure for Chamaecyparis obtusa was developed after co-cultivation of embryogenic tissues with disarmed Agrobacterium tumefaciens strain C58/pMP90, which harbours the sgfp (synthetic green fluorescent protein) visual reporter and nptII (neomycin phoshotransferase II) selectable marker genes. The highest transformation frequency was 22.5 independent transformed lines per dish (250 mg embryogenic tissue) following selection on kanamycin medium. Transgenic plantlets were regenerated through the maturation and germination of somatic embryos. The intensity of GFP fluorescence, observed under a fluorescence microscope, varied from very faint to relatively strong, depending on the transgenic line or part of the transgenic plant. The integration of the genes into the genome of regenerated plantlets was confirmed by Southern blot analysis.
Editor: Germany: Springer Nature B.V
Idioma: Inglês

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Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtusa Sieb. et Zucc

Taniguchi, T ; Kurita, M ; Ohmiya, Y ; Kondo, T

Germany: Springer Nature B.V

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