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Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Lardenois, Aurélie ; Liu, Yuchen ; Walther, Thomas ; Chalmel, Frédéric ; Evrard, Bertrand ; Granovskaia, Marina ; Chu, Angela ; Davis, Ronald W. ; Steinmetz, Lars M. ; Primig, Michael

Proceedings of the National Academy of Sciences - PNAS, 2011-01, Vol.108 (3), p.1058-1063 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6
Autor: Lardenois, Aurélie ; Liu, Yuchen ; Walther, Thomas ; Chalmel, Frédéric ; Evrard, Bertrand ; Granovskaia, Marina ; Chu, Angela ; Davis, Ronald W. ; Steinmetz, Lars M. ; Primig, Michael
Assuntos: Antisense ; Biological Sciences ; Cell cycle ; Cell division ; Cells ; Deoxyribonucleic acid ; Development ; Development Biology ; Diploids ; Diploidy ; DNA ; DNA biosynthesis ; Epigenesis, Genetic ; Epigenesis, Genetic - genetics ; Epigenesis, Genetic - physiology ; Exoribonucleases ; Exoribonucleases - genetics ; Exoribonucleases - metabolism ; Exosome Multienzyme Ribonuclease Complex ; exosomes ; Flow Cytometry ; Gametogenesis ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; Gene Expression Regulation, Fungal - genetics ; Genes ; Haploidy ; Life Sciences ; Meiosis ; Meiosis - physiology ; Messenger RNA ; Mitosis ; Models, Biological ; non-coding RNA ; Oligonucleotides ; Oligonucleotides - genetics ; Promoters ; Proteins ; Regulatory sequences ; Replication ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA, Messenger ; RNA, Messenger - genetics ; RNA, Untranslated ; RNA, Untranslated - genetics ; RNA, Untranslated - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Saccharomycetales ; Sporulation ; Transcription ; Waves ; Yeast ; Yeasts
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2011-01, Vol.108 (3), p.1058-1063
Notas: ObjectType-Article-1
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PMCID: PMC3024698
Author contributions: L.S. and M.P. designed research; Y.L., T.W., and B.E. performed research; F.C., M.G., A.C., R.W.D., and L.S. contributed new reagents/analytic tools; A.L. and F.C. analyzed data; and A.L. and M.P. wrote the paper.
Contributed by Ronald W. Davis, November 8, 2010 (sent for review September 22, 2010)
1A.L. and Y.L. contributed equally to this work.
Descrição: Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

Lardenois, Aurélie ; Liu, Yuchen ; Walther, Thomas ; Chalmel, Frédéric ; Evrard, Bertrand ; Granovskaia, Marina ; Chu, Angela ; Davis, Ronald W. ; Steinmetz, Lars M. ; Primig, Michael

United States: National Academy of Sciences

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