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Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent‐based lignocellulose fractionation and soaking in aqueous ammonia

Rollin, Joseph A ; Zhu, Zhiguang ; Sathitsuksanoh, Noppadon ; Zhang, Y.‐H. Percival

Biotechnology and bioengineering, 2011-01, Vol.108 (1), p.22-30 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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Título:
Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent‐based lignocellulose fractionation and soaking in aqueous ammonia
Autor: Rollin, Joseph A ; Zhu, Zhiguang ; Sathitsuksanoh, Noppadon ; Zhang, Y.‐H. Percival
Assuntos: Ammonia ; Ammonia - metabolism ; Aqueous solutions ; Bioengineering ; Biofuel production ; biofuels ; Biological and medical sciences ; Biomass ; biomass pretreatment ; Biotechnology ; Biotechnology - methods ; Cellulase ; Cellulases - metabolism ; Cellulose ; cellulose accessibility to cellulase ; cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) ; Energy ; Enzymes ; Fractionation ; Fundamental and applied biological sciences. Psychology ; Glucan ; Hydrolysis ; Industrial applications and implications. Economical aspects ; Lignin ; Lignin - metabolism ; lignin removal ; Panicum - chemistry ; Panicum - metabolism ; Pretreatment ; Serum Albumin, Bovine - metabolism ; Soaking ; soaking in aqueous ammonia (SAA) ; Solvents ; Trichoderma - enzymology
É parte de: Biotechnology and bioengineering, 2011-01, Vol.108 (1), p.22-30
Notas: http://dx.doi.org/10.1002/bit.22919
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ark:/67375/WNG-0579TNXP-Q
ArticleID:BIT22919
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Descrição: While many pretreatments attempt to improve the enzymatic digestibility of biomass by removing lignin, this study shows that improving the surface area accessible to cellulase is a more important factor for achieving a high sugar yield. Here we compared the pretreatment of switchgrass by two methods, cellulose solvent‐ and organic solvent‐based lignocellulose fractionation (COSLIF) and soaking in aqueous ammonia (SAA). Following pretreatment, enzymatic hydrolysis was conducted at two cellulase loadings, 15 filter paper units (FPU)/g glucan and 3 FPU/g glucan, with and without BSA blocking of lignin absorption sites. The hydrolysis results showed that the lignin remaining after SAA had a significant negative effect on cellulase performance, despite the high level of delignification achieved with this pretreatment. No negative effect due to lignin was detected for COSLIF‐treated substrate. SEM micrographs, XRD crystallinity measurements, and cellulose accessibility to cellulase (CAC) determinations confirmed that COSLIF fully disrupted the cell wall structure, resulting in a 16‐fold increase in CAC, while SAA caused a 1.4‐fold CAC increase. A surface plot relating the lignin removal, CAC, and digestibility of numerous samples (both pure cellulosic substrates and lignocellulosic materials pretreated by several methods) was also developed to better understand the relative impacts of delignification and CAC on glucan digestibility. Biotechnol. Bioeng. 2011; 108:22-30.
Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglês

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Increasing cellulose accessibility is more important than removing lignin: A comparison of cellulose solvent‐based lignocellulose fractionation and soaking in aqueous ammonia

Rollin, Joseph A ; Zhu, Zhiguang ; Sathitsuksanoh, Noppadon ; Zhang, Y.‐H. Percival

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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