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Facilitated Substrate Channeling in a Self-Assembled Trifunctional Enzyme Complex

You, Chun ; Myung, Suwan ; Zhang, Y.-H. Percival

Angewandte Chemie (International ed.), 2012-08, Vol.51 (35), p.8787-8790 [Periódico revisado por pares]

Weinheim: WILEY-VCH Verlag

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Título:
Facilitated Substrate Channeling in a Self-Assembled Trifunctional Enzyme Complex
Autor: You, Chun ; Myung, Suwan ; Zhang, Y.-H. Percival
Assuntos: Aldolase ; Biocatalysis ; Channeling ; Cohesion ; enzyme cascades ; Enzymes ; Fructose ; Fructose-Bisphosphatase - chemistry ; Fructose-Bisphosphatase - metabolism ; Fructose-Bisphosphate Aldolase - chemistry ; Fructose-Bisphosphate Aldolase - metabolism ; Kinetics ; metabolon ; Pictures ; Protein Structure, Tertiary ; Proteins ; self-assembly ; Substrate Specificity ; Temperature ; Triose-Phosphate Isomerase - chemistry ; Triose-Phosphate Isomerase - metabolism
É parte de: Angewandte Chemie (International ed.), 2012-08, Vol.51 (35), p.8787-8790
Notas: ArticleID:ANIE201202441
This work was supported partially by the DOE BioEnergy Science Center (BESC) and DOE ARPA-E Petro project. This work was also partially supported by the College of Agriculture and Life Sciences Bioprocessing and Biodesign Research Center at Virginia Tech.
istex:1240C192A6897E0DD8EB22BFF1E88BA1AD220798
ark:/67375/WNG-8457PGMD-F
DOE BioEnergy Science Center
BESC
This work was supported partially by the DOE BioEnergy Science Center (BESC) and DOE ARPA‐E Petro project. This work was also partially supported by the College of Agriculture and Life Sciences Bioprocessing and Biodesign Research Center at Virginia Tech.
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USDOE Office of Science (SC), Biological and Environmental Research (BER)
Descrição: Three enzymes, triosephosphate isomerase (orange in picture), aldolase (cyan), and fructose 1,6‐bisphosphatase (purple), which contained dockerins (red), self‐assembled into a static trifunctional enzyme complex through interaction with a mini‐scaffoldin protein consisting of three different cohesins (green). The synthetic enzyme complex exhibited an enhanced reaction rate compared to the noncomplexed three‐enzyme mixture at the same enzyme concentration.
Editor: Weinheim: WILEY-VCH Verlag
Idioma: Inglês

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Facilitated Substrate Channeling in a Self-Assembled Trifunctional Enzyme Complex

You, Chun ; Myung, Suwan ; Zhang, Y.-H. Percival

Weinheim: WILEY-VCH Verlag

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